tailieunhanh - Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome | BioMed Central World Journal of Surgical Oncology Research Real time PCR analyses of expression of E-cadherin alpha- beta-and gamma-catenin in human breast cancer for predicting clinical outcome Amit Goyal Tracey A Martin Robert E Mansel and Wen G Jiang Open Access Address Department of Surgery School of Medicine Cardiff University Cardiff UK Email Amit Goyal - goyala@ Tracey A Martin - MartinTA1@ Robert E Mansel - manselre@ Wen G Jiang - jiangw@ Corresponding author Published II June 2008 Received 24 February 2008 World Journal of Surgical Oncology 2008 6 56 doi 1477-7819-6-56 Accepted 11 June 2008 This article is available from http content 6 1 56 2008 Goyal et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background The E-cadherin catenin system acts as an invasion suppressor of epithelial malignancies. However it is debatable whether expression of E-cadherin or catenins is a useful prognostic marker in invasive breast cancer. Methods We measured the expression of E-cadherin and catenins a- P- Y-catenin in human breast carcinomas using real time quantitative polymerase chain reaction Q-PCR and investigated whether the expression levels were associated with known tumour variables or patient survival median follow-up months . RNA from frozen sections of breast tissue tumour n 124 background normal tissue n 33 was reverse transcribed quantified and analysed by Q-PCR with results expressed as number of copies of transcript 50 ng RNA. Results There was no statistically significant difference in the expression of E-cadherin and catenins a- P- Y-catenin in the 33 .

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