tailieunhanh - Báo cáo y học: "Effect of a small molecule inhibitor of nuclear factor-κB nuclear translocation in a murine model of arthritis and cultured human synovial cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Effect of a small molecule inhibitor of nuclear factor-κB nuclear translocation in a murine model of arthritis and cultured human synovial cells. | Available online http content 7 6 R1348 Research article Effect of a small molecule inhibitor of nuclear factor-KB nuclear translocation in a murine model of arthritis and cultured human synovial cells Kyoko Wakamatsu1 Toshihiro Nanki2 Nobuyuki Miyasaka2 Kazuo Umezawa3 and Tetsuo Kubota1 Department of Microbiology and Immunology Tokyo Medical and Dental University Graduate School of Health Sciences Tokyo Japan 2Department of Medicine and Rheumatology Tokyo Medical and Dental University Graduate School Tokyo Japan 3Department of Applied Chemistry Keio University Kanagawa Japan Corresponding author Tetsuo Kubota Received 1 Jun 2005 Revisions requested 30 Jun 2005 Revisions received 30 Aug 2005 Accepted 2 Sep 2005 Published 30 Sep 2005 Arthritis Research Therapy 2005 7 R1348-R1359 DOI ar1834 This article is online at http content 7 6 R1348 2005 Wakamatsu et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract A small cell-permeable compound dehydroxymethylepoxyquinomicin DHMEQ does not inhibit phosphorylation and degradation of IkB inhibitor of nuclear factor-KB NF-kB but selectively inhibits nuclear translocation of activated NF-kB. This study aimed to demonstrate the antiarthritic effect of this novel inhibitor of the NF-kB pathway in vivo in a murine arthritis model and in vitro in human synovial cells. Collagen-induced arthritis was induced in mice and after onset of arthritis the mice were treated with DHMEQ 5 mg kg body weight per day . Using fibroblast-like synoviocyte FLS cell lines established from patients with rheumatoid arthritis RA NF-kB activity was examined by electrophoretic mobility shift assays. The .