tailieunhanh - Báo cáo y học: "Tumour necrosis factor activates the mitogen-activated protein kinases p38α and ERK in the synovial membrane in vivo"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Tumour necrosis factor activates the mitogen-activated protein kinases p38α and ERK in the synovial membrane in vivo. | Available online http content 7 5 R1140 Open Access Research article Tumour necrosis factor activates the mitogen-activated protein kinases p38a and ERK in the synovial membrane in vivo Birgit Gortz1 2 Silvia Hayer1 Birgit Tuerck1 Jochen Zwerina1 Josef S Smolen1 and Georg Schett1 Division of Rheumatology Department of Internal Medicine III University of Vienna Vienna Austria institute of Pathology University of Giessen Giessen Germany Corresponding author Georg Schett Received 9 May 2005 Revisions requested 14 Jun 2005 Revisions received 27 Jun 2005 Accepted 28 Jun 2005 Published 28 Jul 2005 Arthritis Research Therapy 2005 7 R1140-R1147 DOI ar1797 This article is online at http content 7 5 R1140 2005 Gortz et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Tumour necrosis factor TNF is considered to be a major factor in chronic synovial inflammation and is an inducer of mitogen-activated protein kinase MAPK signalling. In the present study we investigated the ability of TNF to activate MAPKs in the synovial membrane in vivo. We studied human TNF transgenic mice - an in vivo model of TNF-induced arthritis - to examine phosphorylation of extracellular signal-regulated kinase ERK c-Jun amino terminal kinase JNK and p38MAPKa in the inflamed joints by means of immunoblot and immunohistochemistry. In addition the effects of systemic blockade of TNF IL-1 and receptor activator of nuclear factor-kB RANK ligand on the activation of MAPKs were assessed. In vivo overexpression of TNF induced activation of p38MAPKa and ERK in the synovial membrane whereas activation of JNK was less pronounced and rarely observed on immunohistochemical .