tailieunhanh - Báo cáo y học: "dentification of blood biomarkers of rheumatoid arthritis by transcript profiling of peripheral blood mononuclear cells from the rat collagen-induced arthritis model"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: dentification of blood biomarkers of rheumatoid arthritis by transcript profiling of peripheral blood mononuclear cells from the rat collagen-induced arthritis model. | Available online http content 8 1 R28 Research article Identification of blood biomarkers of rheumatoid arthritis by transcript profiling of peripheral blood mononuclear cells from the rat collagen-induced arthritis model Jianyong Shou1 2 Christopher M Bull1 Li Li1 Hui-Rong Qian3 Tao Wei1 Shuang Luo1 Douglas Perkins1 Patricia J Solenberg1 Seng-Lai Tan4 Xin-Yi Cynthia Chen4 Neal W Roehm5 Jeffrey A Wolos1 and Jude E Onyia1 1Integrative Biology Lilly Research Laboratories Indianapolis Indiana USA 2Angiogenesis and Tumor Microenvironment Biology Lilly Research Laboratories Indianapolis Indiana USA 3Statistics Lilly Research Laboratories Indianapolis Indiana USA 4Cancer Inflammation and Cell Survival Lilly Research Laboratories Indianapolis Indiana USA 5Platform CFARS Lilly Research Laboratories Indianapolis Indiana USA Corresponding author Jianyong Shou shou@ Received 28 Sep 2005 Revisions requested 25 Nov 2005 Revisions received 7 Dec 2005 Accepted 9 Dec 2005 Published 10 Jan 2006 Arthritis Research Therapy 2006 8 R28 doi ar1 883 This article is online at http content 8 1 R28 2006 Shou et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Rheumatoid arthritis RA is a chronic debilitating autoimmune disease that results in joint destruction and subsequent loss of function. To better understand its pathogenesis and to facilitate the search for novel RA therapeutics we profiled the rat model of collagen-induced arthritis CIA to discover and characterize blood biomarkers for RA. Peripheral blood mononuclear cells PBMCs were purified using a Ficoll gradient at various time points after type II collagen immunization for RNA preparation. Total RNA .