tailieunhanh - Báo cáo y học: "Shared expression of phenotypic markers in systemic sclerosis indicates a convergence of pericytes and fibroblasts to a myofibroblast lineage in fibrosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Shared expression of phenotypic markers in systemic sclerosis indicates a convergence of pericytes and fibroblasts to a myofibroblast lineage in fibrosis. | Available online http content 7 5 R1113 Research article Shared expression of phenotypic markers in systemic sclerosis indicates a convergence of pericytes and fibroblasts to a myofibroblast lineage in fibrosis Vineeth S Rajkumar1 Kevin Howell1 Katalin Csiszar2 Christopher P Denton1 Carol M Black1 and David J Abraham1 Centre for Rheumatology Connective Tissue Disease Department of Medicine Royal Free Campus University College London London UK 2Cardiovascular Research Center John A Burns School of Medicine University of Hawaii Honolulu HI USA Corresponding author David J Abraham Received 17 May 2005 Accepted 24 Jun 2005 Published 21 Jul 2005 Arthritis Research Therapy 2005 7 R1113-R1123 DOI 1 ar1790 This article is online at http content 7 5 R1113 2005 Rajkumar et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract The mechanisms by which mierovaseular damage leads to dermal fibrosis in diffuse cutaneous systemic sclerosis dcSSc are unclear. We hypothesized that microvascular pericytes constitute a cellular link between microvascular damage and fibrosis by transdifferentiating into myofibroblasts. We used a combination of immunohistochemistry and double immunofluorescence labelling of frozen skin biopsies taken from normal and dcSSc patients to determine whether a phenotypic link between pericytes and myofibroblasts exists in dcSSc. Using a-smooth muscle actin the ED-A splice variant of fibronectin ED-A FN and Thy-1 to identify myofibroblasts we demonstrated the presence of myofibroblasts in fibrotic dcSSc skin. Myofibroblasts were totally absent from control skin atrophic stage dcSSc skin and non-lesional skin. Using double