tailieunhanh - Báo cáo y học: "Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro. | Available online http content 7 5 R927 Research article Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro Duncan E Crombie1 Muhammed Turer1 Beltzane Biurrun Zuasti1 Bayden Wood2 Don McNaughton2 Kutty Selva Nandakumar3 Rikard Holmdahl3 Marie-Paule Van Damme1 and Merrill J Rowley1 department of Biochemistry and Molecular Biology Monash University Clayton Victoria Australia 2Centre for Biospectroscopy and School of Chemistry Monash University Clayton Victoria Australia 3Medical Inflammation Research Lund University Lund Sweden Corresponding author Merrill J Rowley Received 6 Jan 2005 Revisions requested 9 Feb 2005 Revisions received 12 Apr 2005 Accepted 10 May 2005 Published 6 Jun 2005 Arthritis Research Therapy 2005 7 R927-R937 DOI 86 ar1 766 This article is online at http content 7 5 R927 2005 Crombie et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Certain monoclonal antibodies mAbs to type II collagen CII induce arthritis in vivo after passive transfer and have adverse effects on chondrocyte cultures and inhibit self assembly of collagen fibrils in vitro. We have examined whether such mAbs have detrimental effects on pre-existing cartilage. Bovine cartilage explants were cultured over 21 days in the presence of two arthritogenic mAbs to CII CIIC1 or M2139 a non-arthritogenic mAb to CII CIIF4 or a control mAb GAD6 . Penetration of cartilage by mAb was determined by immunofluorescence on frozen sections and correlated with changes to the extracellular matrix and chondrocytes by morphometric analysis of sections stained with toluidine blue. The effects of mAbs

TÀI LIỆU LIÊN QUAN
TỪ KHÓA LIÊN QUAN
crossorigin="anonymous">
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.