tailieunhanh - Báo cáo y học: "Synovial microparticles from arthritic patients modulate chemokine and cytokine release by synoviocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Synovial microparticles from arthritic patients modulate chemokine and cytokine release by synoviocytes. | Available online http content 7 3 R536 Research article Synovial microparticles from arthritic patients modulate chemokine and cytokine release by synoviocytes René J Berckmans1 Rienk Nieuwland1 Maarten C Kraan2 Marianne CL Schaap1 Desirée Pots2 Tom JM Smeets2 Augueste Sturk1 and Paul P Tak2 Department of Clinical Chemistry Academic Medical Center University of Amsterdam Amsterdam The Netherlands department of Clinical Immunology and Rheumatology Academic Medical Center University of Amsterdam Amsterdam The Netherlands Corresponding author René J Berckmans Received 13 Oct 2004 Revisions requested 1 Nov 2004 Revisions received 26 Jan 2005 Accepted 2 Feb 2005 Published 1 Mar 2005 Arthritis Research Therapy 2005 7 R536-R544 DOI 86 ar1 706 This article is online at http content 7 3 R536 2005 Berckmans et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Synovial fluid from patients with various arthritides contains procoagulant cell-derived microparticles. Here we studied whether synovial microparticles modulate the release of chemokines and cytokines by fibroblast-like synoviocytes FLS . Microparticles isolated from the synovial fluid of rheumatoid arthritis RA and arthritis control AC patients n 8 and n 3 respectively were identified and quantified by flow cytometry. Simultaneously arthroscopically guided synovial biopsies were taken from the same knee joint as the synovial fluid. FLS were isolated cultured and incubated for 24 hours in the absence or presence of autologous microparticles. Subsequently cell-free culture supernatants were collected and concentrations of monocyte chemoattractant protein-1 MCP-1 IL-6 IL-8 granulocyte