tailieunhanh - Báo cáo y học: "Repression of anti-proliferative factor Tob1 in osteoarthritic cartilage"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Repression of anti-proliferative factor Tob1 in osteoarthritic cartilage. | Available online http content 7 2 R274 Research article Repression of anti-proliferative factor Tobi in osteoarthritic cartilage Mathias Gebauer1 Joachim Saas2 Jochen Haag3 Uwe Dietz2 Masaharu Takigawa4 Eckart Bartnik2 and Thomas Aigner3 1Aventis Pharma Deutschland Functional Genomics Sanofi-Aventis Frankfurt Germany 2Sanofi-Aventis Disease Group Thrombotic Diseases Degenerative Joint Diseases Frankfurt Germany 3Osteoarticular and Arthritis Research Department of Pathology University of Erlangen-Nurnberg Germany 4Department of Biochemistry and Molecular Dentistry Okayama University Graduate School of Medicine and Dentistry Okayama Japan Contributed equally Corresponding author Thomas Aigner Received 10 Aug 2004 Revisions requested 1 Oct 2004 Revisions received 22 Oct 2004 Accepted 19 Nov 2004 Published 11 Jan 2005 Arthritis Res Ther 2005 7 R274-R284 DOI ar1479 2005 Gebauer et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Osteoarthritis is the most common degenerative disorder of the modern world. However many basic cellular features and molecular processes of the disease are poorly understood. In the present study we used oligonucleotide-based microarray analysis of genes of known or assumed relevance to the cellular phenotype to screen for relevant differences in gene expression between normal and osteoarthritic chondrocytes. Custom made oligonucleotide DNA arrays were used to screen for differentially expressed genes in normal n 9 and osteoarthritic n 10 cartilage samples. Real-time polymerase chain reaction PCR with gene-specific primers was used for quantification. Primary human adult articular chondrocytes and .

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