tailieunhanh - Báo cáo y học: "Molecular profile of synovial fibroblasts in rheumatoid arthritis depends on the stage of proliferation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Molecular profile of synovial fibroblasts in rheumatoid arthritis depends on the stage of proliferation. | Available online http 4 5 R8 Research article Molecular profile of synovial fibroblasts in rheumatoid arthritis depends on the stage of proliferation Kimio Masuda1 Riako Masuda1 Michel Neidhart1 Beat R Simmen2 Beat A Michel3 Ulf Muller-Ladner4 Renate E Gay1 and Steffen Gay1 Center of Experimental Rheumatology and WHO Collaborating Center for Molecular Biology and Novel Therapeutic Strategies for Rheumatic Diseases Department of Rheumatology University Hospital Zurich Gloriastrasse 25 CH-8091 Zurich Switzerland 2Upper Extremity and Handsurgery Schulthess Clinic Lengghalde 2 CH-8008 Zurich Switzerland 3Department of Rheumatology University Hospital Zurich Gloriastrasse 25 CH-8091 Zurich Switzerland 4Department of Internal Medicine I University of Regensburg D-93042 Regensburg Germany Corresponding author Steffen Gay e-mail Received 15 March 2002 Revisions received 6 June 2002 Accepted 21 June 2002 Published 17 July 2002 Arthritis Res 2002 4 R8 2002 Masuda et al. licensee BioMed Central Ltd Print ISSN 1465-9905 Online ISSN 1465-9913 Abstract The aim of this study was to explore the molecular profile of proliferating rheumatoid arthritis synovial fibroblasts RA-SF . Total RNA was extracted from two cultures of RA-SF low-density LD proliferating cells and high-density HD nonproliferating cells and suppression subtractive hybridization was performed to compare differential gene expression of these two cultures. Subtracted cDNA was subcloned and nucleotide sequences were analyzed to identify each clone. Differential expression of distinct clones was confirmed by semiquantitative RT-PCR. The expression of certain genes in synovial tissues was examined by in situ hybridization. In both LD and HD cells 44 clones were upregulated. Of the 88 total clones 46 were identical to sequences that have previously been characterized. Twenty- nine clones were identical to cDNAs that have been identified but with unknown functions so far and