tailieunhanh - Báo cáo y học: "Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren’s syndrome"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren’s syndrome. | Available online http 4 4 R4 Research article Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjogren s syndrome Annett M Jacobi1 Arne Hansen2 Olaf Kaufmann3 Axel Pruss4 Gerd R Burmester1 Peter E Lipsky5 and Thomas Dorner1 1 Department of Internal Medicine Rheumatology and Clinical Immunology Charite University Hospital Berlin Germany 2Outpatients Department Charite University Hospital Berlin Germany institute of Pathology Charite University Hospital Berlin Germany institute of Transfusion Medicine Charite University Hospital Berlin Germany 5NIAMS National Institutes of Health Bethesda Maryland USA Corresponding author Thomas Dorner e-mail Received 19 February 2002 Revisions received 7 May 2002 Accepted 13 May 2002 Published 11 June 2002 Arthritis Res 2002 4 R4 2002 Jacobi et al. licensee BioMed Central Ltd Print ISSN 1465-9905 Online ISSN 1465-9913 Abstract Patients with Sjogren s syndrome SS have characteristic lymphocytic infiltrates of the salivary glands. To determine whether the B cells accumulating in the salivary glands of SS patients represent a distinct population and to delineate their potential immunopathologic impact individual B cells obtained from the parotid gland and from the peripheral blood were analyzed for immunglobulin light chain gene rearrangements by PCR amplification of genomic DNA. The productive immunglobulin light chain repertoire in the parotid gland of the SS patient was found to be restricted showing a preferential usage of particular variable lambda chain genes VX2E and variable kappa chain genes VkA27 . Moreover clonally related VL chain rearrangements were identified namely VkA27-Jk5 and VkA19-Jk2 in the parotid gland and VX1C-JX3 in the parotid gland and the peripheral blood. Vk and VX rearrangements from the parotid gland exhibited a significantly elevated mutational frequency compared with those from the peripheral blood P .