tailieunhanh - Báo cáo y học: "Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease."

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease. | Available online http content 4 4 261 Review Use of soluble MHC class II peptide multimers to detect antigen-specific T cells in human disease Jerome R Bill and Brian L Kotzin Departments of Medicine and Immunology University of Colorado Health Sciences Center and National Jewish Medical and Research Center Denver Colorado USA Corresponding author Jerome R Bill e-mail Received 20 November 2001 Revisions received 1 February 2002 Accepted 6 February 2002 Published 28 February 2002 Arthritis Res 2002 4 261-265 2002 BioMed Central Ltd Print ISSN 1465-9905 Online ISSN 1465-9913 Abstract Most techniques that identify antigen-specific T cells are dependent on the response of these cells to the relevant antigen in culture. Soluble multimers of MHC molecules when occupied with the same peptide will bind selectively to T cells specific for that MHC peptide complex. Techniques to produce fluorescent MHC class II peptide multimers have recently been developed. These reagents provide a method to facilitate detection and isolation of antigen-specific CD4 T cells and they represent a new research tool to study these cells in patients with immune-mediated diseases. Keywords flow cytometry MHC class II MHC peptide multimer T cell T-cell receptor Background CD4 and CD8 T cells through their T-cell receptors TCRs recognize peptides bound to MHC class II and class I molecules respectively. The peptide derived from the protein antigen and the restricting MHC molecule are both critical for specific binding of the TCR. Until recently the identification or quantitation of antigen-specific T cells was possible only by assaying for their function. Classically a population of T cells was cocultured with antigen and antigen presenting cells which express surface MHC molecules. Several days later tritiated thymidine was added to the culture and antigen-induced T-cell proliferation was quantitated by the amount of incorporated thymidine. .