tailieunhanh - Báo cáo y học: "Differential binding of chemokines to macrophages and neutrophils in the human inflamed synovium"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Differential binding of chemokines to macrophages and neutrophils in the human inflamed synovium. | Available online http content 4 3 209 Research article Differential binding of chemokines to macrophages and neutrophils in the human inflamed synovium Angela M Patterson Caroline Schmutz Scott Davis Lucy Gardner Brian A Ashton and Jim Middleton Leopold Muller Arthritis Research Centre Centre for Science and Technology in Medicine Keele University at Robert Jones and Agnes Hunt Orthopaedic Hospital Oswestry Shropshire UK. Correspondence Dr Jim Middleton LMARC Robert Jones and Agnes Hunt Orthopaedic Hospital Oswestry Shropshire SY10 7AG UK. Tel 44 0 1691 404149 fax 44 0 1691 404170 e-mail Middleton1@ Received 30 March 2001 Revisions requested 25 May 2001 Revisions received 31 October 2001 Accepted 12 December 2001 Published 31 January 2002 Arthritis Res 2002 4 209-214 2002 Patterson et al. licensee BioMed Central Ltd Print ISSN 1465-9905 Online ISSN 1465-9913 Abstract In chronic inflammatory foci such as the rheumatoid joint there is enhanced recruitment of phagocytes from the blood into the tissues. Chemokines are strongly implicated in directing the migration of these cells although little is known regarding the chemokine receptors that could mediate their chemotaxis into the joint tissue. Therefore the objective of the study was to identify chemokine binding sites on macrophages and neutrophils within the rheumatoid synovium using radiolabeled ligand binding and in situ autoradiography. Specific binding sites for CCL3 macrophage inflammatory protein-1a CCL5 RANTES CCL2 monocyte chemoattractant protein-1 and CXCL8 IL-8 were demonstrated on CD68 macrophages in the subintimal and intimal layers. The number and percentage of intimal cells that bound chemokines were greater in inflamed regions compared to noninflamed regions. The intensity of intimal binding varied between chemokines with the rank order CCL3 CCL5 CCL2 CXCL8. Neutrophils throughout the synovium bound CXCL8 but did not show any signal for binding CCL2 CCL3 or CCL5. .