tailieunhanh - Báo cáo y học: "Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue. | Available online http content 4 3 201 Research article Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue Shiva Shahrara1 Michael V Volin1 Matthew A Connors1 G Kenneth Haines2 and Alisa E Koch1 3 1 Department of Medicine Northwestern University Medical School IL USA 2Department of Pathology Northwestern University Medical School IL USA 3Veterans Administration Chicago Health Care System Lakeside Division Chicago IL USA Correspondence Alisa E Koch MD Northwestern University Medical School Department of Medicine Section of Rheumatology 303 East Chicago Avenue Ward Building 3-315 Chicago Illinois 60611 USA. Tel 1 312 503 1963 fax 1 312 503 0994 e-mail ae-koch@ Received 6 March 2001 Revisions requested 10 April 2001 Revisions received 5 November 2001 Accepted 27 November 2001 Published 16 January 2002 Arthritis Res 2002 4 201-208 2002 Shahrara et al. licensee BioMed Central Ltd Print ISSN 1465-9905 Online ISSN 1465-9913 Abstract Angiopoietins Ang are vascular endothelial cell-specific growth factors that play important roles principally during the later stages of angiogenesis. We have compared the distribution of the receptor tyrosine kinase Tie and the Ang ligands in synovial tissues from normal subjects and those with rheumatoid arthritis RA and osteoarthritis OA . Immunohistochemical analysis was used to determine the expression of Ang-1 Ang-2 Tie1 and Tie2 in synovial tissue of normal subjects and those with RA and OA. Ang-1 Ang-2 Tie1 and Tie2 mRNA and protein expression were quantified in synovial tissues and RA synovial tissue fibroblasts with real-time reverse transcription polymerase chain reaction and western blot analysis. In RA Ang-1 positive immunostaining on lining cells macrophages and endothelial cells was significantly higher than in OA and normal synovial tissue. The expression pattern of Ang-2 in synovial tissue was similar in RA and OA whereas the Ang-2 .