tailieunhanh - Báo cáo lâm nghiệp: "Occurrence of foliar nitrate reductase activity not induced by nitrate in symbiotic nitrogen-fed black alder (Alnus glutinosa)"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Occurrence of foliar nitrate reductase activity not induced by nitrate in symbiotic nitrogen-fed black alder (Alnus glutinosa). | 777s Ann. Sci. For. 1989 46 suppl. 777s-781s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Occurrence of foliar nitrate reductase activity not induced by nitrate in symbiotic nitrogen-fed black alder Alnus glutinosa s. Benamar1 G. Pizelle1 and G. Thiéry2 Laboratoire de Physiologìe Végétale et Forestiere Faculte des Sciences BP 239 54506 Vandoeuvre-lès-Nancy Cedex and 2Physiologie Végétale ENSAIA 54500 Vandoeuvre-lès-Nancy France Introduction Black alder Alnus glutinosa L. Gaertn. acquires nitrogen from its environment by symbiotic nitrogen fixation within its actinorhizas and by uptake of combined nitrogen from the soil solution. NOg represents the major form of combined nitrogen in alder soils which possess a high capacity for nitrification Bollen and Lu 1968 . It is well established that black alder has the ability to reduce NO3 in both roots and leaves Pizelle and Thiéry 1974 1986 . The present study was performed on young black alders grown under axenic or non-axenic conditions and supplied with nitrate or nitrate-free nutrient solution. The objectives were to 1 evaluate the effect of nitrogen source and plant age on leaf nitrate reductase NR activity measured in vivo 2 verify that leaf NR activity was not due to an artifact of microbial origin 3 examine the relationship between plant growth and leaf NR activity. Materials and Methods Young black alders were grown in a growth chamber lighVdark cycle temperature and RH 16 8 h 25 18 c and 60 80 respectively photon flux density 200 zE m-2-h-1 from Metalarc Sylvania lamps. Axenic and non-axenic plants were grown on perlite in test tubes and on a vermiculite-sand mixture v v respectively. Nodulation was obtained if necessary by inoculation with a pure Frankia suspension in axenic cultures and with an actinorhiza suspension in non-axenic cultures. The nodulated plants were grown on an N-free solution 4 mM NaNO3 was added to this solution to supply the nitrate-fed plants. Leaf NR activity was determined .

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