tailieunhanh - Báo cáo lâm nghiệp: "Micropropagation of Eucalyptus dunnii Maid"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Micropropagation of Eucalyptus dunnii Maid. | 140s Ann. Sci. For. 1989 46 suppl. 140s-144s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Micropropagation of Eucalyptus dunnii Maid. . Cortezzi Graẹa and s. Mendes Centro Nacional de Pesquisa de Florestas EMBRAPA Curitiba PR Brazil Introduction Among the Eucalyptus recommended for reforestation in the southern region of Brazil Eucalyptus dunnii Maid has been the most promising due to its rapid growth stem straightness and frost tolerance. The establishment of tree improvement programs and extensive plantations however have been restrained due to both low seed production Graọa 1987 and low rooting capacity when propagated by stem cuttings. The high multiplication rates which can be obtained with micropropagation techniques can assist in overcoming these problems as plant production is rapidly increased. Although several eucalypt species have been in vitro propagated Hartney 1982 work with E. dunnii has not been reported. This paper describes a micropropagation technique for rejuvenated E. dunnii. Materials and Methods Nodal segments of E. dunnii about 1 cm long containing one node from greenhouse grown plants derived from stem cuttings were used as explants. Explants were disinfected by 30 min immersion in g l-1 Benomyl immediately followed by soaking for 5 min in v v commercial detergent and for 15 min in 1 sodium hypochlorite. The disinfectants were removed by 3 successive rinses in autoclaved and bidistilled water. In the multiplication stage explants were cultured on MS medium Murashige and Skoog 1962 containing the following substances mg l myoinositol 100 nicotinic acid pyridoxine-HCI thiamine-HCI 0 5 glycine 2 adenine sulfate 20 sucrose 30 000 and Difco Bacto-agar 6 000 . Growth regulator treatments were 6-benzylaminopurine BAP at and mg- - combined with indole-3-butyric acid IBA at and mgl-1. The pH was adjusted to . Cultures were maintained under 16 h 8 h lighVdark photoperiod and 25 2 C after 30 .

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