tailieunhanh - Báo cáo lâm nghiệp: "A micropropagation system for Eucalyptus dunnii x Eucalyptus sp"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: A micropropagation system for Eucalyptus dunnii x Eucalyptus sp. | 136s Ann. Sci. For. 1989 46 suppl. 136s-139s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA A micropropagation system for Eucalyptus dunniix Eucalyptus sp. M. Fantini and . Cortezzi Graẹa2 1 Klabin do Parana Agro-Florestal Telẽmaco Borda PR and 2 Centro Nacional de Pesquisa de Florestas CNPF-EMBRAPA PR Brazil Introduction A Eucalyptus dunnii hybrid was first noticed during seedling production in 1984. This spontaneous hybrid originated from a seed production area of E. dunnii and its characteristics such as leaf color shape wax content and stem color were distinct from the type. Although the hybrid growth potential cannot yet be determined early tree selection revealed 2 important features 1 tolerance to frost comparable to that of the parental species which is a major factor to consider in establishing Eucalyptus in the southern region of Brazil 2 the hybrid can be easily propagated by stem cuttings as opposed to E. dunnii. While this method is successful in vitro propagation would reduce propagation stock requirements and would also be a rapid method for mass clonal propagation. The development of a system to micropropagate Eucalyptus dunnii X Eucalyptus sp. was the objective of this study. Materials and Methods Nodal segments of E. dunnii X Eucalyptus sp. were collected from rooted cuttings actively growing in an open greenhouse. After leaf removal segments were surface-sterilized in 1 NaCIO plus 2 drops of Tween-20 100 ml for 15 min followed by 3 rinses in autoclaved distilled and deionized water. The basal medium for initiation consisted of Murashige and Skoog 1962 salts plus vitamins as described by Gamborg and Wetter 1975 3 sucrose with BAP benzylaminopurine and IBA indole butyric acid at mg-H. The pH of the medium was adjusted to prior to the addition of 6 g-l-1 of Bacto-agar. At the multiplication stage the medium combinations of BAP and KIN kinetin and mg-H and IBA and mg-H were used. For the shoot .

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