tailieunhanh - Báo cáo lâm nghiệp: "Polyamines and ethylene during of Prunus avium L. in vitro rooting"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Polyamines and ethylene during of Prunus avium L. in vitro rooting. | 124s Ann. Sci. For. 1989 46 suppl. 124s-126s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Polyamines and ethylene during in vitro rooting of Prunus avium L. s. Biondi1 and N. Bagni2 1 Azienda Regionale Foreste dellEmilia-Romagna Bologna and 2 Dipartimento di Biologia . Università di Bologna Bologna Italy Introduction Polyamines are widely occurring organic polycations now recognized as plant growth substances. They appear to be involved in cell division to delay senes-cense and usually accompany active growth and metabolism Bagni ef al. 1982 . Although a requirement for polyamines has been postulated in some in vitro morphogenetic processes a full understanding of their role in organogenesis has yet to come. The interaction between ethylene and auxin is a well-known phenomenon. Our primary interest in studying the action of ethylene in adventitious root formation stems from auxin usually being the key factor in root induction. Secondly since ethylene and polyamines share a common precursor S-adenosylmethionine in their biosynthetic pathways there is some evidence for a possible interrelationship between them. The aim of the present work was to begin examining the changes in the endogenous content of the polyamines putrescine PUT spermidine SPD and spermine SPM and in the ethylene production accompanying the in vitro rooting of Prunus avium shoots. In addition the effects of externally supplied polyamines in particular SPM of the ethylene precursor 1 -aminocyclopropane-1 -carboxylic acid ACC and of some inhibitors of polyamine and ethylene biosyntheses namely a-difluoromethylornithine DFMO a-di-fluoromethylarginine DFMA dicyclohexylamine DOHA methylglyoxal-bis-guanyl-hydrazone MGBG and aminoetho-xyvinylglycine AVG and aminooxyacetic acid AOA were examined. Materials and Methods The experimental material is part of a routine micropropagation program for selected clones of p. avium L. The establishment of bud cultures and the shoot multiplication and