tailieunhanh - Báo cáo lâm nghiệp: "In vitro propagation of Prosopis P. cineraria and P. juliflora) species"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: In vitro propagation of Prosopis P. cineraria and P. juliflora) species. | 110s Ann. Sci. For. 1989 46 suppl. 11 Os-112s Forest Tree Physiology E. Dreyer etal. eds. Elsevier INRA In vitro propagation of Prosopis species P. chilensis p cineraria and p. juliflora . Batchelor D. Yao . Koehler and . Harris Department of Biological Sciences Coventry Polytechnic Priory Street Coventry CV1 5FB . Introduction The genus Prosopis has attracted considerable interest for forestry in arid areas although normally propagated by seed vegetative propagation of selected Proso-pis plants from variable populations and from natural hybrids may also be desirable. Propagation by cuttings has been reported . Felker and Clark 1981 . The use of tissue culture techniques to regenerate plants from nodal explants has also been reported for p. cineraria Goyal and Arya 1984 p. tamarugo and p. chilensis Jordan and Balbao 1985 and p. alba Tabone eta . 1986 . In this study tissue culture media were evaluated for root initiation shoot proliferation and shoot growth of p. chilensis p. cineraria and p. juliflora. Materials and Methods Explants with 1 or 2 nodes were taken from the youngest 6 nodes of the main stem and branches of 3-12 mo old greenhouse-grown stock plants. Explants were surface sterilized in 70 industrial methylated spirit for 1 min and 5 v v sodium hypochlorite for 5-10 min and immersed in an anti-oxidant solution 100 mg l citric acid 50 mg l ascorbic acid 100 mg l polyvinyl pyrrolidone for 15 min. Murashige and Skoog medium was used with 8 g l agar 30 g l sucrose g l glutamine and 81 combinations of plant hormones including kinetin K mg l benzylamino purine BA mg l indole acetic acid IAA 1-10 mg l indole butyric acid IBA 1-15 mg l and naphthalene acetic acid NAA 1-15 mg l . Between 4 and 16 explants were placed on each medium and incubated at 25 c with a 16 h photoperiod and a photon flux density of 65-200 íE m2 for 51 d. Results Without hormones results for rooting percentage mean number of shoots explant node and mean