tailieunhanh - Báo cáo lâm nghiệp: " Somatic embryos from callus of von Salix viminalis"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Somatic embryos from callus of von Salix viminalis . | 108s Ann. Sci. For. 1989 46 suppL 108s-109s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Somatic embryos from callus of Salix viminalis L. L. Gronroos s. von Arnold and T. Ericsson Department of Physiological Botany Uppsala University Uppsala Sweden Introduction This report is a summary of a manuscript which is in preparation and will be published elsewhere. Somatic embryogenesis in pistil callus of basket willow Salix vimi-nalis L. is reported. Tissue culture techniques could become useful for breeding purposes if plants could be propagated via somatic embryogenesis. In order to facilitate identification of embryogenic callus obtained from Salix in the future the embryogenic callus and the other callus types obtained are described morphologically anatomically and ultrastructurally. Materials and Methods Floral buds of a female Salix viminalis L. clone 78195 were collected during the non-growing season from field-grown plants provided by the Swedish Energy Forestry Pro ect. The buds were surface-sterilized for 10-15 min in calcium hypochlorite and rinsed in sterile water. After removal of the bud scales the excised catkins were briefly covered with 70 ethanol and then rinsed 3 times in sterile water. Pistils were isolated from the catkins and cultured in darkness at 25 c on MS medium Murashige and Skoog 1962 containing 5 pM 2 4-dichloro-phenoxy-acetic acid and pM benzyladenine and solidified with Gelrite gellan gum. The callus produced was subcultured every 3 wk. Different callus types were isolated from each other. Established calluses were transferred to hormone-free medium and cultured in dim light 14-20 pE-m-2-s-i . Somatic embryos produced were cultured on medium with pM abscisic acid ABA . Results Calluses started to proliferate from the pistils within 2 wk. After ca 6 wk 2 main types of calluses could be separated from each other. Type 1 was a soft callus with a smooth wet-looking surface. It was composed of long thin tubular