tailieunhanh - Báo cáo lâm nghiệp: "Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase. | 64s Ann. Sci. For. 1989 46 suppl. 64s-66s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase M. Villar1 M. Gaget2 and c. Dumas2 ỵiNRA Station dAmélioration des Arbres Forestiers Ardon F-45160 Olivet and 2Laboratoire de Reconnaissance Cellulaire et Amélioration des Plantes INRA 23879 Universitó Lyon I 43 bd 11-Novembre-1918 F-69622 Villeurbanne Cedex France Introduction Hybridization in Populus breeding programs is limited by sexual incompatibility barriers whose cellular and molecular mechanisms are not yet known. In an attempt to understand the nature of interspecific incompatibility in Populus we have explored the interactions between male and female partners pollen-pistil in compatible and incompatible crosses p. nigra female X p. nigra male and p. nigra female X p. alba male Villar 1987 . Materials and Methods p. nigra and p. alba branches were obtained from the INRA Forestry station in Orleans France . Pollen was collected and stored in closed vials at -18 c. Kinetics of pollen tube growth in vivo were studied in growth chambers 20 C . Pollen tubes in the stylar tissues were visualized using the aniline blue fluorescence method ABF method Dumas and Knox 1983 . Isoelectric focusing IEF of pollinic and stig-matic proteins gel preparation focusing condi tions silver nitrate staining and homogenates of pollinated stigma were performed according to the procedures of Villar et al. 1988 . Each of the 2 crosses was represented by 3 series of stigmatic extracts in accordance with the growth kinetics and 20 h after pollination . Protein patterns of these extracts were compared on a single gel. Glycoprotein revelation concanavalin A-binding proteins on IEF polyacrylamide gel was adapted from Hawkes 1982 -Galactosidase visualization was optimized from the protocol of Singh and Knox 1985 . Results and Discussion Kinetics of pollen tube growth visualization using .