tailieunhanh - Báo cáo khoa học: "A new method for the histochemical localization of laccase in Rhus verniciflua Stokes"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "A new method for the histochemical localization of laccase in Rhus verniciflua Stokes. | 856s Ann. Sei. For. 1989 46 suppl. 856S-859S Forest Tree Physiology E. Dreyer etal. eds. Elsevier INRA A new method for the histochemical localization of laccase in Rhus verniciflua Stokes . Li Department of Forestry and Natural Resources University of Edinburgh The King s Buildings Mayfield Road Edinburgh EH9 3JU Scotland . Introduction There has been great interest in laccase for over 100 yr since Yoshida first detected this copper-containing enzyme in 1883. Laccase EC is found in the latex of species of Rhus and is responsible for the oxidation of phenol urushiol which is contained in the latex with production of the black resinous lacquer Bonner 1950 . Besides Rhus laccase is also found in numerous other woody plants including Aesculus sp Prunus persica Acer pseudoplatanus and many species of the Anacardiaceae family and in a number of fungi as well as in some herbaceous plants Bonner 1950 Butt 1980 Mayer and Harel 1979 Bligny and Douce 1983 Although it is moderately widely distributed in higher plants and there have been many reports concerning its biochemical and biophysical properties laccase has benefitted from little or no investigation and in the literature there are no speculations as to its physiological function in the secretory ducts of Rhus. One of the main difficulties encountered in studying its physiological function may be the inactivation of the enzyme during extraction and purification its substrate urushiol is apparently necessary for maintaining it in an undenatured state Guo 1981 Laccase can be demonstrated in vitro by means of biochemical methods such as spectrophotometry oxygen-absorbance and polarography from the liquid lacquer or from a cell-cultured suspension Guo 1981 Gan and Gan 1983 Bligny and Douce 1983 . For the purpose of studying its physiological function in the lacquer tree a simple permanent staining technique for in situ laccase fixation has been developed which permits its enzymatic activity to be maintained .

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