tailieunhanh - Báo cáo khoa học: "CCL25-CCR9 interaction modulates ovarian cancer cell migration, metalloproteinase expression, and invasion"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: CCL25-CCR9 interaction modulates ovarian cancer cell migration, metalloproteinase expression, and invasion | Johnson et al. World Journal of Surgical Oncology 2010 8 62 http content 8 1 62 WORLD JOURNAL OF SURGICAL ONCOLOGY RESEARCH Open Access CCL25-CCR9 interaction modulates ovarian cancer cell migration metalloproteinase expression and invasion Erica L Johnson1 Rajesh Singh1 Shailesh Singh1 Crystal M Johnson-Holiday1 William E Grizzle2 Edward E Partridge3 James W Lillard Jr1 Abstract Background Ovarian carcinoma OvCa is the most lethal gynecological malignancy among women and its poor prognosis is mainly due to metastasis. Chemokine receptor CCR9 is primarily expressed by a small subset of immune cells and its only natural ligand CCL25 is largely expressed in the thymus which involutes with age. Other than the thymus CCL25 is expressed by the small bowel. Interactions between CCL25 and CCR9 have been implicated in leukocyte trafficking to the small bowel a frequent metastatic site for OvCa cells. The current study shows OvCa tissue and cells significantly express CCR9 which interacts with CCL25 to support carcinoma cell migration and invasion. Methods RT-PCR and flow cytometry techniques were used to quantify the expression CCR9 by OvCa cells. OvCa tissue microarrays TMA was used to confirm CCR9 expression in clinical samples. The Aperio ScanScope scanning system was used to quantify immunohistochemical staining. Cell invasion and migration assays were performed using cell migration and matrigel invasion chambers. Matrix metalloproteinase MMP mRNAs were quantified by RT-PCR and active MMPs were quantified by ELISA. Results Our results show significantly p higher expression of CCR9 by mucinous adenocarcinoma papillary serous carcinoma and endometriod ovarian carcinoma cases than compared to non-neoplastic ovarian tissue. Furthermore CCR9 expression was significantly elevated in OvCa cell lines OVCAR-3 and CAOV-3 in comparison to normal adult ovarian epithelial cell mRNA. OvCa cells showed higher migratory and invasive potential towards chemotactic .