tailieunhanh - Báo cáo khoa học: "The combined effect of erythropoietin and granulocyte macrophage colony stimulating factor on liver regeneration after major hepatectomy in rats"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The combined effect of erythropoietin and granulocyte macrophage colony stimulating factor on liver regeneration after major hepatectomy in rats | Vassiliou et al. World Journal of Surgical Oncology 2010 8 57 http content 8 1 57 RESEARCH WORLD JOURNAL OF SURGICAL ONCOLOGY Open Access The combined effect of erythropoietin and granulocyte macrophage colony stimulating factor on liver regeneration after major hepatectomy in rats loannis Vassiliou 1 Evangelos Lolis1 Constantinos Nastos1 Aliki Tympa1 Theodosios Theodosopoulos1 Nikolaos Dafnios1 George Fragulidis1 Matrona Frangou2 Agathi Kondi-Pafiti2 and Vassilios Smyrniotis1 Abstract Background The liver presents a remarkable capacity for regeneration after hepatectomy but the exact mechanisms and mediators involved are not yet fully clarified. Erythropoietin EPO and Granulocyte-Macrophage Colony Stimulating Factor GM-CSF have been shown to promote liver regeneration after major hepatectomy. Aim of this experimental study is to compare the impact of exogenous administration of EPO GM-CSF as well as their combination on the promotion of liver regeneration after major hepatectomy. Methods Wistar rats were submitted to 70 major hepatectomy. The animals were assigned to 4 experimental groups a control group n 21 that received normal saline an EPO group n 21 that received EPO 500 IU kg a GM-CSF group n 21 that received 20 mcg kg of GM-CSF and a EPO GMCSF group n 21 which received a combination of the above. Seven animals of each group were killed on the 1st 3rd and 7th postoperative day and their remnant liver was removed to evaluate liver regeneration by immunochemistry for PCNA and Ki 67. Results Our data suggest that EPO and GM-CSF increases liver regeneration following major hepatectomy when administered perioperatively. EPO has a more significant effect than GM-CSF p . When administering both the effect of EPO seems to fade as EPO and GM-CSF treated rats have decreased regeneration compared to EPO administration alone p . Conclusion EPO GM-CSF and their combination enhance liver regeneration after hepatectomy in rats when administered .