tailieunhanh - Báo cáo khoa học: Macrocypins, a family of cysteine protease inhibitors from the basidiomycete Macrolepiota procera

A new family of cysteine protease inhibitors from the basidiomyceteMac-rolepiota procera has been identified and the family members have been termed macrocypins. These macrocypins are encoded by a family of genes that is divided into five groups with more than 90% within-group sequence identity and 75–86% between-group sequence identity. | ễFEBS Journal Macrocypins a family of cysteine protease inhibitors from the basidiomycete Macrolepiota procera Jerica Sabotic1 Tatjana Popovic 1 Vida Puizdar2 and Joze Brzin1 1 Department of Biotechnology Jozef Stefan Institute Jamova 39 Ljubljana Slovenia 2 Department of Biochemistry and Molecular and StructuralBiology JoZef Stefan Institute Jamova 39 Ljubljana Slovenia Keywords basidiomycetes clitocypin cysteine protease mycocypin protease inhibitor Correspondence J. Sabotic Department of Biotechnology JoZef Stefan Institute Jamova 39 1000 Ljubljana Slovenia Tel 386 1 477 3754 Fax 386 1 477 3594 E-mail Note The nucleotide sequences reported in this paper have been deposited in the DDBI EMBL GenBank databases under accession numbers FJ495239 FJ495240 FJ495241 FJ495242 FJ495243 FJ495244 FJ495245 FJ495246 FJ495247 FJ495248 FJ495249 FJ495250 FJ548751 and FJ548752 Received 25 March 2009 revised 22 May 2009 accepted 9 June 2009 doi A new family of cysteine protease inhibitors from the basidiomycete Mac-rolepiota procera has been identified and the family members have been termed macrocypins. These macrocypins are encoded by a family of genes that is divided into five groups with more than 90 within-group sequence identity and 75-86 between-group sequence identity. Several differences in the promoter and noncoding sequences suggest regulation of macrocypin expression at different levels. High yields of three different recombinant macrocypins were produced by bacterial expression. The sequence diversity was shown to affect the inhibitory activity of macrocypins the heterologously expressed macrocypins belonging to different groups showing differences in their inhibitory profiles. Macrocypins are effective inhibitors of papain and cysteine cathepsin endopeptidases and also inhibit cathepsins B and H which exhibit both exopeptidase and endopeptidase activities. The cysteine protease legumain is inhibited by macrocypins