tailieunhanh - Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation

The Cys2His2-type zinc finger transcription factor serum amyloid A activa-ting factor 1 [SAF-1, also known as MAZ (myc-associated zinc finger protein) or Pur-1 (purine binding factor-1)] plays an important role in regu-lation of a variety of inflammation-responsive genes. | SAF-3 a novel splice variant of the SAF-1 MAZ Pur-1 family is expressed during inflammation Alpana Ray1 Srijita Dhar1 Arvind Shakya1 Papiya Ray1 Yasunori Okada2 and Bimal K. Ray1 1 Department of Veterinary Pathobiology University of Missouri Columbia MO USA 2 Department of Pathology Schoolof Medicine Keio University Tokyo Japan Keywords gene expression inflammation SAF-1 MAZ Pur-1 splice variant transcription factor Correspondence B. K. Ray Department of Veterinary Pathobiology University of Missouri 124 Connaway Hall Columbia MO 65211 USA Fax 1 573 884 5414 Tel 1 573 882 4461 E-mail rayb@ A. Ray Department of Veterinary Pathobiology University of Missouri 126 Connaway Hall Columbia MO 65211 USA Fax 1 573 884 5414 Tel 1 573 882 6728 E-mail rayal@ Database The sequence for MAZ genomic DNA has been submitted to the Genbank database under the accession numbers D89880. Received 6 January 2009 revised 12 May 2009 accepted 5 June 2009 doi The Cys2His2-type zinc finger transcription factor serum amyloid A activating factor 1 SAF-1 also known as MAZ myc-associated zinc finger protein or Pur-1 purine binding factor-1 plays an important role in regulation of a variety of inflammation-responsive genes. An SAF-2 splice variant acting as a negative regulator of SAF-1 was identified previously and the present study reports the identification of a novel SAF-3 splice variant that is expressed during inflammation. SAF-3 mRNA isolated from a cDNA library produced from IL-1b-induced cells originates from a previously unknown first coding exon and thereby contains a unique N-termi-nal domain but shares the same six zinc finger DNA-binding domains as present in SAF-1. In addition a negatively functioning domain present at the N-terminus of SAF-1 and SAF-2 is spliced out in SAF-3. The expression of SAF-3 is very low in normal tissues and in cells grown under normal conditions. However RT-PCR analysis of mRNAs from cytokine and .