tailieunhanh - Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells

ATP, via purinergic P2X receptors, acts as a neurotransmitter and modula-tor in both the central and peripheral nervous systems, and is also involved in many biological processes, including cell proliferation, differentiation and apoptosis. Previously, we have reported that P2X7 receptor inhibition promotes axonal growth and branching in cultured hippocampal neurons. | Ca2 calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells Rosa Gomez-Villafuertes1 Ana del Puerto2 3 4 Miguel Diaz-Hernandez1 4 Diego Bustillo5 Juan I. Diaz-Hernandez1 Paula G. Huerta1 Antonio R. Artalejo5 Juan J. Garrido2 3 4 and Ma Teresa Miras-Portugal1 1 Departamento de Bioquimica y Biologia Molecular Facultad de Veterinaria Universidad Complutense de Madrid Spain 2 Centro de Biologia Molecular Severo Ochoa CSIC-UAM Madrid Spain 3 Departamento de Neurobiologia Celular Molecular y delDesarrollo Instituto Cajal CSIC Madrid Spain 4 Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas CIBERNED Spain 5 Departamento de Toxicologia y Farmacologia Facultad de Veterinaria Universidad Complutense de Madrid Spain Keywords brilliant blue G Ca2 calmodulin-dependent kinase II neuritogenesis neuroblastoma P2X7 receptor Correspondence M. T. Miras-Portugal Departamento de Bioquimica y Biologia Molecular IV Facultad de Veterinaria Universidad Complutense de Madrid Av. Puerta de Hierro s n 28040 Madrid Spain Fax 34 91 3943909 Tel 34 91 3943894 E-mail mtmiras@ Received 13 May 2009 revised 25 June 2009 accepted 22 July 2009 doi ATP via purinergic P2X receptors acts as a neurotransmitter and modulator in both the central and peripheral nervous systems and is also involved in many biological processes including cell proliferation differentiation and apoptosis. Previously we have reported that P2X7 receptor inhibition promotes axonal growth and branching in cultured hippocampal neurons. In this article we demonstrate that the P2X7 receptor negatively regulates neurite formation in mouse Neuro-2a neuroblastoma cells through a Ca2 calmodulin-dependent kinase II-related mechanism. Using both molecular and immunocytochemical techniques we characterized the presence of endogenous P2X1 P2X3 P2X4 and P2X7 subunits in these cells. Of these the .