tailieunhanh - Báo cáo khoa học: The carbohydrate-binding module family 20 – diversity, structure, and function

Starch-active enzymes often possess starch-binding domains (SBDs) medi-ating attachment to starch granules and other high molecular weight sub-strates. SBDs are divided into nine carbohydrate-binding module (CBM) families, and CBM20 is the earliest-assigned and best characterized family. | REVIEW ARTICLE The carbohydrate-binding module family 20 - diversity structure and function Camilla Christiansen1 2 3 Maher Abou Hachem2 Stefan Janecek4 5 Anders Viks0-Nielsen3 Andreas Blennow1 and Birte Svensson2 1 VKR Research Centre Pro-Active Plants Department of Plant Biology and Biotechnology Faculty of Life Sciences University of Copenhagen Frederiksberg Denmark 2 Enzyme and Protein Chemistry Department of Systems Biology TechnicalUniversity of Denmark Lyngby Denmark 3 Novozymes A S Bagsvaerd Denmark 4 Institute of Molecular Biology Slovak Academy of Sciences Bratislava Slovakia 5 Department of Biotechnology Faculty of NaturalSciences University of SS Cyriland Methodius Trnava Slovakia Keywords a-glucan amylolytic enzymes glucan molecular recognition starch-binding domain starch metabolism water dikinase Correspondence M. Abou Hachem Enzyme and Protein Chemistry Department of Systems Biology TechnicalUniversity of Denmark Soltofts Plads Building 224 DK-2800 Kgs. Lyngby Denmark Fax 45 4588 6307 Tel 45 4525 2732 E-mail maha@ Received 22 May 2009 accepted 17 July 2009 doi Starch-active enzymes often possess starch-binding domains SBDs mediating attachment to starch granules and other high molecular weight substrates. SBDs are divided into nine carbohydrate-binding module CBM families and CBM20 is the earliest-assigned and best characterized family. High diversity characterizes CBM20s which occur in starch-active glycoside hydrolase families 13 14 15 and 77 and enzymes involved in starch or glycogen metabolism exemplified by the starch-phosphorylating enzyme glucan water dikinase 3 from Arabidopsis thaliana and the mammalian glycogen phosphatases laforins. The clear evolutionary relatedness of CBM20s to CBM21s CBM48s and CBM53s suggests a common clan hosting most of the known SBDs. This review surveys the diversity within the CBM20 family and makes an evolutionary comparison with CBM21s CBM48s and CBM53s discussing .