tailieunhanh - Candida infections detection and epidemiology - part 6

Do đó, phải chú ý đến dòng chảy làm việc. Phòng thí nghiệm nên được chia nhỏ trong "bản sao không ',' bản sao thấp và các khu vực làm việc cao bản sao, nếu có thể được trang bị với quá áp suất (không sao chép, tức là làm sạch các khu vực) hoặc không có áp lực | False-positive results and contaminations amplification techniques reveal high amounts of DNA so called high copy corresponding with high risk . Therefore attention must be paid to the work-flow. Laboratories should be subdivided in no copy low copy and high copy working areas if possible equipped with overpressure no copy . clean areas or underpressure high copy . contaminated areas . These working areas can comprise separate rooms biosafety hoods or for this purpose designed cabinets26 36 54. Also flowcabinets can be used in which the direction of flow ensures that aerosols are pushed down to the base of the cabinet away from the top of the reaction tubes51. Activities in which no DNA is involved preparation of PCR-mixes should be performed first after which activities can be build up via low copy extraction of low copy nucleic acids adding template to premixes to high copy operations analysis of amplificationproducts or other sources of high copy DNA . Subsequently the no- and low copy work area should not be entered again that particular day. Furthermore it is recommended to aliquot reagents in an area that is free of nucleic acids and to use different freezers and refrigerators for storage of reagents and samples. Providing every work area with separate sets of supplies and equipment like racks pipettes test tube holders centrifuges etc. substantially reduces the risk of carry-over contamination. Appropriate disposable clothing should be present in each room and these should be changed frequently . gloves masks coats mob caps goggles 34 36 79. Additionally it is recommended to clean the working areas equipment and literally everything which is routinely touched by hands like doorknobs handles of freezers telephones etc. on a regular base. Sodium hypochlorite and HCl are mentioned as cleaning agents for work areas and equipment. Prince et al. recommend a concentration of sodium hypochlorite w v 5 min for fragments as small as 76 bp59. This .