tailieunhanh - Báo cáo khoa học: Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids

Par j 1 and Par j 2 proteins are the two major allergens inParietaria juda-ica pollen, one of the main causes of allergic diseases in the Mediterranean area. Each of them contains eight cysteine residues organized in a pattern identical to that found in plant nonspecific lipid transfer proteins. | ễFEBS Journal Par j 1 and Par j 2 the two major allergens in Parietaria judaica bind preferentially to monoacylated negative lipids Roberto Gonzalez-Rioja1 Juan A. Asturias1 Alberto Martinez1 Felix M. Gobi2 3 and Ana Rosa Viguera2 1 Research and Development Department Bial-Aristegui Bilbao Spain 2 Unidad de Biofisica CSIC-UPV EHU Leioa Spain 3 Departamento de Bioquimica Universidad delPais Vasco Leioa Spain Keywords cavity volume CD lipid binding lipid transfer proteins pyrene fluorescence Correspondence A. R. Viguera Unidad de Biofisica CSIC-UPV EHU Barrio Sarriena s n 48940 Leioa Spain Fax 34 946 01 3360 Tel 34 946 01 3191 E-mail gbbviria@ Received 5 November 2008 revised 5 January 2009 accepted 19 January 2009 doi Par j 1 and Par j 2 proteins are the two major allergens in Parietaria judaica pollen one of the main causes of allergic diseases in the Mediterranean area. Each of them contains eight cysteine residues organized in a pattern identical to that found in plant nonspecific lipid transfer proteins. The 139- and 102-residue recombinant allergens corresponding respectively to Par j 1 and Par j 2 refold properly to fully functional forms whose immunological properties resemble those of the molecules purified from the natural source. Molecular modeling shows that despite the lack of extensive primary structure homology with nonspecific lipid transfer proteins both allergens contain a hydrophobic cavity suited to accommodate a lipid ligand. In the present study we present novel evidence for the formation of complexes of these natural and recombinant proteins from Parietaria pollen with lipidic molecules. The dissociation constant of oleyl-lyso-phos-phatidylcholine is pM for recombinant Par j 1 whereas pyrene-dodecanoic acid shows a much higher affinity with a dissociation constant of approximately 1 M for both recombinant proteins as well as for the natural mixture. Lipid binding does not alter the secondary .

• Báo cáo khoa học
• medical reports
• thesis report
• traditional medicine
• immune system
• Antimicrobial
• biosynthesis
• Olea europaea
• Vitamin E biosynthesis
• Gene expression
• Tocopherol biosynthesis genes
• Synthesized in plants
• Mango malformation
• Transcriptomic analysis of Brassinosteroid biosynthesis
• Brassinosteroid biosynthesis
• Signaling pathway genes
• Malformed tissues
• Mango variety amrapalli
• Tartary buckwheat
• Jasmonate ZIM domain proteins
• Yeast two hybrid
• Anthocyanin biosynthesis
• Flavonoid biosynthesis
• Plant Biology
• Camellia sinensis
• Integrated transcriptomics
• Metabolomics analysis
• Theanine biosynthesis
• Scientific reports
• biochemistry
• molecular Biology
• Trypanosoma
• medical research
• breast cancer
• medicine
• Structural Biology
• intermediate cells
• metabolites
• biochemical studies
• health education
• Glutathionylspermidine is an intermediate formed in the biosynthesis of trypanothione
• an essential metabolite in defence against chemical and oxi dative stress in the Kinetoplastida
• The kinetic mechanism for glutathionyl spermidine synthetase (EC 6
• 8) from Crithidia fasciculata (CfGspS) obeys a rapid equilibrium random ter ter model with kinetic constants KGSH= 609lm
• KSpd= 157lmand KATP= 215lm
• scientific research
• oxidation
• cytoplasm
• biological cells
• Streptomyces
• molecular
• analysis of cytoplasmic
• hemoglobin
• Ebook Biochemistry
• Synthesizing themolecules of life
• The biosynthesis of amino acids
• The biosynthesis of membrane lipids
• Responding to environmental changes
• Sensory systems
• Tạp chí Đại học công nghiệp thực phẩm
• Effect of plant oils on mycelial biomass production
• Biosynthesis and antioxidants of exopolysaccharide
• Immunomodulatory and antitumor
• Demonstrated that exopolysaccharides
• Vietnam Journal of Science and Technology
• Rice bran oil on mycelial biomass production
• Biosynthesis and bioactivities of polysaccharides
• Ophiocordyceps sinensis fungus
• Rice bran oil
• Daucus carota
• MYB 6 and Restriction digestion
• Regulating Anthocyanin biosynthesis
• GFP Tagged MYB 6 Gene
• Aimed to clone GFP
• BMC Plant Biology
• P appendiculatum
• Flavonoids biosynthesis
• Basic helix loop helix
• Transcription factors
• Pyrus bretschneideri
• Stone cells
• Lignin biosynthesis
• Transcriptomic analysis
• Cinnamoyl CoA reductase
• Solanum melongena L
• High temperature
• BMC Biotechnology
• Tribolium castaneum
• RNA interference
• Chitin biosynthesis pathway
• Gene function
• Gossypol biosynthesis
• Plant grafting
• Organ culture
• Gene expression profiling
• RNA seq
• Ester biosynthesis
• Transcriptional regulation
• Ripening fruit
• Amaryllidaceae alkaloid
• Pathogenesis related protein 10
• Alkaloid biosynthesis
• Narcissus pseudonarcissus
• Norcoclaurine synthase
• Inflorescence architecture
• BRs biosynthesis
• Panicle architecture
• PMM1 transcripts
• Tissue acidification
• Litchi chinensis
• Rubber biosynthesis
• Parthenium argentatum
• Hevea plantations