tailieunhanh - Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells

The binding sequences for signal transducer and activator of transcription (STAT) and activator protein 1 have been found in the promoter region of the angiotensinogen gene. We examined whether the elements for activator protein 1 and STAT5B function in angiotensinogen gene activation induced by angiotensin II in vascular smooth muscle cells. | Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells Mei Han Ai-Ying Li Fang Meng Li-Hua Dong Bin Zheng Hai-Juan Hu Lei Nie and Jin-Kun Wen Department of Biochemistry and Molecular Biology Hebei MedicalUniversity Shijiazhuang China Keywords activator protein-1 angiotensinogen gene regulation signal transducer and activator of transcription-5 vascular smooth muscle cells Correspondence . Wen Department of Biochemistry and Molecular Biology No. 361 Zhongshan East Road Shijiazhuang 050017 China Fax 86 311 86266180 Tel 86 311 8626 5563 E-mail wjk@ Received 29 October 2008 revised 29 December 2008 accepted 12 January 2009 doi The binding sequences for signal transducer and activator of transcription STAT and activator protein 1 have been found in the promoter region of the angiotensinogen gene. We examined whether the elements for activator protein 1 and STAT5B function in angiotensinogen gene activation induced by angiotensin II in vascular smooth muscle cells. Stimulation with angiotensin II increased the level of angiotensinogen mRNA by in vascular smooth muscle cells. The increased level of angiotensinogen mRNA occurred with concurrent elevations in the levels of STAT5B and c-Jun phosphorylation after stimulation with angiotensin II. Likewise angiotensin II resulted in similar enhancements of the DNA-binding activity of STAT5B and c-Jun in angiotensin II-induced angiotensinogen expression. Notably the STAT5B-DNA complex interacted with the c-Jun-DNA complex by forming a stable quaternary complex in angiotensin II-induced angiotensinogen expression. Our findings support a model in which co-operative interaction of STAT5B and activator protein 1 bound to the the promoter region provides maximal activation of angiotensinogen expression by angiotensin II in vascular smooth