tailieunhanh - Báo cáo khoa học: The b-N-acetylglucosaminidases NAG1 and NAG2 are essential for growth of Trichoderma atroviride on chitin

The chitinolytic enzyme machinery of fungi consists of chitinases andb-N-acetylglucosaminidases. These enzymes are important during the fungal life cycle for degradation of exogenous chitin, which is the second most abun-dant biopolymer, as well as fungal cell-wall remodelling. | ỊFEBS Journal The p-N-acetylglucosaminidases NAG1 and NAG2 are essential for growth of Trichoderma atroviride on chitin Ruben Lopez-Mondejar Valentina Catalanof Christian P. Kubicek and Verena Seidl Research Area Gene Technology and Applied Biochemistry Institute of ChemicalEngineering Vienna University of Technology Austria Keywords chitin degradation chitinases mycoparasitism N-acetylglucosaminidases Trichoderma atroviride Correspondence V. Seidl Research Area Gene Technology and Applied Biochemistry Institute of ChemicalEngineering Vienna University of Technology Getreidemarkt 9 166-5 1060 Vienna Austria Fax 43 1 58801 17299 Tel 43 1 58801 17227 E-mail vseidl@ Website http pg Seidl Present addresses Department of SoilWater Conservation and Organic Waste Management Centro de Edafologia y Biologia Aplicada delSegura CEBAS-CSIC PO Box 164 30100 Espi-nardo Murcia Spain fDepartment of Tree Science Entomology and Plant Pathology G. Scaramuzzi Plant Pathology Section Faculty of Agriculture University of Pisa Via delBorghetto 80 I-56124 Pisa Italy Received 10 June 2009 revised 26 June 2009 accepted 13 July 2009 doi The chitinolytic enzyme machinery of fungi consists of chitinases and b-N-acetylglucosaminidases. These enzymes are important during the fungal life cycle for degradation of exogenous chitin which is the second most abundant biopolymer as well as fungal cell-wall remodelling. In addition involvement of chitinolytic enzymes in the lysis of the host cell wall in mycoparasitic Trichoderma spp. has been reported. In view of the fact that fungi have on average 15-20 chitinases but only two b-N-acetylglucosami-nidases the question arises how important the latter enzymes actually are for various aspects of chitin degradation. In this study the role of two b-N-acetylglucosaminidases NAG1 and NAG2 was analysed in the myco-parasitic fungus Trichoderma atroviride. No b-N-acetylglucosaminidase activity

TÀI LIỆU MỚI ĐĂNG
crossorigin="anonymous">
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.