tailieunhanh - Báo cáo khoa học: A large complex mediated by Moc1, Moc2 and Cpc2 regulates sexual differentiation in fission yeast

Sexual differentiation inSchizosaccharomyces pombeis triggered by nutri-ent starvation and is downregulated by cAMP. Screening programs have identified the moc1/sds23, moc2/ded1, moc3andmoc4/zfs1genes as inducers of sexual differentiation, even in the presence of elevated levels of cAMP. | ễFEBS Journal A large complex mediated by Moc1 Moc2 and Cpc2 regulates sexual differentiation in fission yeast Swapan Kumar Paul Yasuo Oowatari and Makoto Kawamukai Department of Applied Bioscience and Biotechnology Shimane University Matsue Japan Keywords fission yeast Moc protein Schizosaccharomyces pombe sexual differentiation translation Correspondence M. Kawamukai Department of Applied Bioscience and Biotechnology Faculty of Life and Environmental Science Shimane University 1060 Nishikawatsu Matsue 6908504 Japan Fax 81 852 32 6092 Tel 81 852 32 6587 E-mail kawamuka@ Received 10 June 2009 revised 2 July 2009 accepted 7 July 2009 doi Sexual differentiation in Schizosaccharomyces pombe is triggered by nutrient starvation and is downregulated by cAMP. Screening programs have identified the moc1Ịsds23 moc2Ịded1 moc3 and moc4 zfs1 genes as inducers of sexual differentiation even in the presence of elevated levels of cAMP. To investigate possible interactions among Moc1 Moc2 Moc3 and Moc4 proteins we first screened for individual Moc-interacting proteins using the yeast two-hybrid system and verified the interactions with other Moc proteins. Using this screening process Cpc2 and Rpl32-2 were highlighted as factors involved in interactions with multiple Moc proteins. Cpc2 interacted with Moc1 Moc2 and Moc3 whereas the ribosomal protein Rpl32-2 interacted with all Moc proteins in the two-hybrid system. Physical interactions of Cpc2 with Moc1 Moc2 and Rpl32-2 and of Rpl32-2 with Moc2 were confirmed by coimmunoprecipitation. In addition using Blue Native PAGE we revealed that each Moc protein exists as a large complex. Overexpression of Moc1 Moc2 Moc3 Moc4 and Rpl32-2 resulted in the efficient induction of a key transcription factor Ste11 suggesting that all proteins tested are positive regulators of Ste11. Considering that Moc2 Ded1 is a general translation factor and that Cpc2 associates with many ribosomal proteins