tailieunhanh - Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans

To elucidate the function of kappa class glutathione transferases (GSTs) in multicellular organisms, their expression and silencing were investigated in Caenorhabditis elegans. In contrast with most vertebrates, which possess only one GST kappagene, two distinct genes encoding GSTK-1 and GSTK-2 are present in theC. elegansgenome. | ễFEBS Journal Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria respectively and are involved in lipid metabolism and respiration in Caenorhabditis elegans Flicii p tít1 2 3 ỵa ít l linKlot4 5 f l91 HinRaiinh1 2 3 liiQÝĨnn RnrÝríìnH-IK lĩnhnl6 PronnniQ Tprf p6 7 8 se eLIL Advlcl ceiet aune auc UoLII lc eiLian ce I ai Ibulo clue Renaud Legouis4 5 and Fabrice Morel1 2 3 1 Inserm U620 Université de Rennes 1 France 2 EA-MDC Universite de Rennes 1 France 3 IFR 140 Rennes France 4 CNRS Centre de Genetique Moleculaire - UPR2167 Gif-sur-Yvette France 5 Universite Paris-Sud Orsay Universite Paris-6 France 6 IFR150 Institut Federatif de Recherche Bio-Medicale de Toulouse Plateau technique de Lipidomique France 7 INSERM U563 Toulouse France 8 Universite Toulouse III PaulSabatier Departement Lipoproteines et Mediateurs Lipidiques IFR150 France Keywords Caenorhabditis elegans fatty acids glutathione transferase kappa mitochondria peroxisomes Correspondence F. Morel INSERM U522 EA MDC Hopital Pontchaillou 35033 Rennes France Fax 33 299540137 Tel 33 299543737 E-mail R. Legouis Centre de Genetique Moleculaire - UPR2167 CNRS Batiment 26 Avenue de la terrasse 91198 Gif-sur-Yvette Cedex France Tel 33 169824374 Fax 33 169824386 E-mail legouis@ Received 28 May 2009 revised 3 July 2009 accepted 7 July 2009 doi To elucidate the function of kappa class glutathione transferases GSTs in multicellular organisms their expression and silencing were investigated in Caenorhabditis elegans. In contrast with most vertebrates which possess only one GST kappa gene two distinct genes encoding GSTK-1 and GSTK-2 are present in the C. elegans genome. The amino acid sequences of GSTK-1 and GSTK-2 share around 30 similarity with the human hGSTK1 sequence and like the human transferase GSTK-1 contains a C-terminal peroxisomal targeting sequence. gstk-1 and gstk-2 genes show distinct developmental and