tailieunhanh - Báo cáo khoa học: "Study of endogenous plant growth Douglas fir II. Gibberellin analysis"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài:"Study of endogenous plant growth Douglas fir II. Gibberellin analysis. | 259s Ann. Sci. For. 1989 46 suppl. 259S-263S Forest Tree Physiology E. Dreyer et al. eds Elsevier INRA study of endogenous plant growth substances in Douglas fir II. Gibberellin analysis p. Doumas1 J. Bianco2 and M. Bonnet-Masimbert1 1 station d Amelioration des Arbres Forestiers INRA Ardon 45160 Olivet and 1 Laboratoire de Physiologie Végétale Université de Nice 06000 Nice France Introduction Flowering in Pinaceae conifers can be brought about by the application of less polar gibberellins GAs especially GA4 7 applied singly or in combination with other plant growth regulators such as naphthyl acetic acid or culture treatments such as high temperature water stress girdling or root-pruning Pharis and Ross 1986 . GAs seem to be essential in the flowering induction strategy. It is therefore important to know the endogenous GAs of a species before trying to interpret any physiological role of endogenously or exogenously applied GAs. The level of endogenous GAs in plant tissues is generally very low 1-10 ng g fresh weight . Consequently selective methods must be used to analyze GAs. One course of action is to use selective GA immunoassays to detect immunoreactive components in high performance liquid chromatography HPLC eluates. Weiler and his coworkers Weiler and Wieczoreck 1981 Aztorn and Weiler 1983a b have shown that immunological analyses of GAs could be effective and promising. We have developed a procedure combining HPLC separation and enzyme-linked immunosorbent assay ELISA which can recognize a limited number of GAs. We have analyzed the effect of flowerinducing treatments on GA levels from juvenile trees. This paper reports preliminary results on the analysis of several GA-like substances in elongating shoots of Douglas fir Pseudotsuga menziesii Mirb. with or without a flower-inducing treatment independent of any flowering response on such juvenile trees. Materials and Methods Plant material Experiments were performed at INRA Orleans France on 4 yr old .