tailieunhanh - Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB

It has been well documented that both endogenous inflammatory mediator advanced glycation end products (AGEs) and exogenous inflammatory inducer lipopolysaccharide play key roles in the initiation and development of inflammatory diseases. However, the combined inflammation-stimulatory effect of AGEs and lipopolysaccharide on endothelial cells, and, further-more, the underlying signal transduction pathways involved, have not been fully elucidated. | Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-KB Jinghua Liu1 Shanchao Zhao1 Jing Tang1 Zhijie Li1 Tianyu Zhong1 Yawei Liu1 Dengyu Chen1 Mingzhe Zhao1 Yusheng Li1 Xiaowei Gong1 Peng Deng1 Jiang H. Wang2 and Yong Jiang1 1 Key Laboratory of FunctionalProteomics of Guangdong Province Department of Pathophysiology Southern MedicalUniversity Guangzhou China 2 Department of Surgery University College Cork Cork University Hospital Cork Ireland Keywords advanced glycation end products cytokines chemokines lipopolysaccharide MAP kinases NF-kB Correspondence J. H. Wang or Y. Jiang Department of Surgery University College Cork Cork University Hospital Cork Ireland Key Laboratory of Functional Proteomics of Guangdong Province Department of Pathophysiology Southern Medical University Guangzhou 510515 China Fax 353 21 4901240 86 20 87277521 Tel 353 21 4901275 86 20 61648231 E-mail yjiang@ Received 17 April2009 revised 17 June 2009 accepted 22 June 2009 doi It has been well documented that both endogenous inflammatory mediator advanced glycation end products AGEs and exogenous inflammatory inducer lipopolysaccharide play key roles in the initiation and development of inflammatory diseases. However the combined inflammation-stimulatory effect of AGEs and lipopolysaccharide on endothelial cells and furthermore the underlying signal transduction pathways involved have not been fully elucidated. We found that in vitro co-stimulation with AGE-human serum albumin HSA and lipopolysaccharide exhibits a synergistic effect on proinflammatory cytokine chemokine interleukin-6 interleukin-8 and monochemoattractant protein-1 production in human umbilical vein endothelial cells. Similar to lipopolysaccharide AGE-HSA stimulation induced mitogen-activated protein .