tailieunhanh - Báo cáo khoa học: "In vitro propagation of interspecific hybrids"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "In vitro propagation of interspecific hybrids. | 155s Ann. Sci. For. 1989 46 suppl. 155s-157s Forest Tree Physiology E. Dreyer etal. eds. Elsevier INRA In vitro propagation of interspecific hybrids in Alnus H. Sbay J. Guillot p. Danthu and D. Prat Laboratoire de Génétique des Populations d Arbres Forestiers ENGRER 14 rue Girardet F-54042 Nancy France Introduction Alnus species show promise for afforestation and wood production particularly on poor soils since they are fast-growing and nitrogen-fixing trees. This allows mixed plantations with benefits to the main accompanying forest species by nitrogen supply. The genus Alnus includes some fast-growing species adapted to various ecological situations Martin 1985 . Genetic improvement programs are being developed to produce effective clonal varieties able to grow under various ecological conditions. Controlled hybridizations intraspecific and interspecific were carried out to obtain improved progenies from which trees will be selected. Field trials show good performance of interspecific hybrids Prat 1988 . The latter should be propagated to confirm their superiority and then be distributed afterwards as selected clones. In vitro micropropagation is applied because of the poor development of cuttings. Materials and Methods Early selection of trees at age 4 yr was carried out in progeny trials 4 progenitor species were used A. glutlnosa A. cordata A. incana and A rubra The best performing and plastic hybrids Prat 1988 were studied A glutinosa X A. incana Gl A rubra XA. glutinosa RG A. cordata X A. glutinosa CG and A. cordata X A. incana Cl . Shoots cut from selected trees were soaked in fungicide Benlate for 24 h and then disinfected with calcium hypochlorite 7 for 10 min and kept on nutritive medium containing sucrose for 1 day. Afterwards shoots were disinfected with a mercuric chloride solution for 10 min. . Nodes were separated in an anti-oxidative solution mM dithiothrei-tol IĨ1M cysteine hydrochloride 2 8 mM citrulline mM sodium ascorbate .

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