tailieunhanh - Báo cáo khoa học: Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms

Talins are large adaptor proteins that link the integrin family of adhesion molecules to F-actin. In vertebrates, there are two talin 1is essential for integrin-mediated cell adhesion; the role oftalin 2 is unclear. Here we report a detailed analysis of mammaliantalin 2. | Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms Emmanuel Debrand Yasmine El Jai Lorraine Spence Neil Bate Uta Praekelt Catrin A. Pritchard Susan J. Monkley and David R. Critchley Department of Biochemistry University of Leicester UK Keywords cytoskeleton integrins spermatogenesis talin 2 talin 2 isoforms Correspondence D. R. Critchley Department of Biochemistry University of Leicester Henry Wellcome Building Lancaster Road Leicester LE1 9HN UK Fax 44 116 229 7018 Tel 44 116 229 7099 E-mail drc@ Re-use of this article is permitted in accordance with the Creative Commons Deed Attribution which does not permit commercial exploitation Received 3 September 2008 revised 27 November 2008 accepted 9 January 2009 doi Talins are large adaptor proteins that link the integrin family of adhesion molecules to F-actin. In vertebrates there are two talin genes. Talin 1 is essential for integrin-mediated cell adhesion the role of talin 2 is unclear. Here we report a detailed analysis of mammalian talin 2. This reveals the existence of a previously unrecognized promoter associated with a CpG island and separated from the first coding exon by numerous alternatively spliced noncoding exons spanning 200 kb. Analysis of a mouse gene trap line shows that this promoter accounts for most of the talin 2 expression in adult tissues. We also demonstrate that testis and kidney express truncated talin 2 isoforms that lack the N-terminal half of the protein and provide evidence for the developmentally regulated expression of the short testis-specific talin 2 isoform in elongating spermatids. Finally we identify four tissue-specific alternative splicing events within the coding region of talin 2. Talin is a large 270 kDa 2540 amino acids dimeric cytoskeletal protein that couples the integrin family of ab heterodimeric cell adhesion molecules to the actin cytoskeleton 1 . It also plays a key role in regulating the