tailieunhanh - Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas

Magnetic resonance spectroscopy studies are often carried out to provide metabolic information on tumour cell metabolism, aiming for increased knowledge for use in anti-cancer treatments. Accordingly, the presence of intense lipid signals in tumour cells has been the subject of many studies aiming to obtain further insight on the reaction of cancer cells to external agents that eventually cause cell death. | ỊFEBS Journal Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas Anna Maria Luciani1 Sveva Grande1 Alessandra Palma1 Antonella Rosi1 Claudio Giovannini2 Orazio Sapora3 Vincenza Viti1 and Laura Guidoni1 1 Dipartimento di Tecnologie e Salute and INFN Gruppo Collegato Sanita Istituto Superiore di Sanita Rome Italy 2 Dipartimento di Sanita Pubblica Veterinaria e Sicurezza Alimentare Istituto Superiore di Sanita Rome Italy 3 Dipartimento di Ambiente e Connessa Prevenzione Primaria Istituto Superiore di Sanita Rome Italy Keywords cell cycle cell metabolism lipids magnetic resonance spectroscopy tumour cell lines Correspondence L. Guidoni Dipartimento di Tecnologie e Salute Istituto Superiore di Sanita 00161 Rome Italy Fax 39 06 4938 7075 Tel 39 06 4990 2804 E-mail guidoni@ Received 5 November 2008 revised 15 December 2008 accepted 22 December 2008 doi Magnetic resonance spectroscopy studies are often carried out to provide metabolic information on tumour cell metabolism aiming for increased knowledge for use in anti-cancer treatments. Accordingly the presence of intense lipid signals in tumour cells has been the subject of many studies aiming to obtain further insight on the reaction of cancer cells to external agents that eventually cause cell death. The present study explored the relationship between changes in neutral lipid signals during cell growth and after irradiation with gamma rays to provide arrest in cell cycle and cell death. Two cell lines from human tumours were used that were differently prone to apoptosis following irradiation. A sub-G1 peak was present only in the radiosensitive HeLa cells. Different patterns of neutral lipids changes were observed in spectra from intact cells either during unperturbed cell growth in culture or after radiation-induced growth arrest. The intensities of triglyceride signals in the spectra from extracted total lipids changed concurrently. The .

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