tailieunhanh - Báo cáo khoa học: Transcription factor specificity protein 1 (SP1) and activating protein 2a (AP-2a) regulate expression of human KCTD10 gene by binding to proximal region of promoter

Shikimate dehydrogenase (SDH) catalyzes the NADPH-dependent reduction of 3-dehydroshikimate to shikimate in the shikimate pathway. In this study, we determined the kinetic properties and crystal structures of Staphylococcus epidermidisSDH (SeSDH) both in its ligand-free form and in complex with shikimate. | ễFEBS Journal Transcription factor specificity protein 1 SP1 and activating protein 2a AP-2a regulate expression of human KCTD10 gene by binding to proximal region of promoter Rushi Liu 1 Aidong Zhou1 Daolong Ren1 Allan He1 Xiang Hu1 Wenfeng Zhang1 Liping Yang1 Mingjun Liu1 Hong Li1 Jianlin Zhou1 Shuanglin Xiang1 and Jian Zhang1 2 1 Key Laboratory of Protein Biochemistry and Development Biology of State Education Ministry of China Hunan Normal university China 2 ModelOrganisms Division Shanghai Second MedicalUniversity China Keywords AP-2a KCTD10 promoter regulatory element SP1 Correspondence J. Zhang Key Laboratory of Protein Biochemistry and Development Biology of State Education Ministry of China College of Life Sciences Hunan NormalUniversity Changsha Hunan 410081 China Fax 86 731 887 2792 Tel 86 731 887 2792 E-mail Zhangjian@ These authors contributed equally to this work Received 25 October 2008 revised 7 December 2008 accepted 11 December 2008 doi Potassium channel tetramerization domain-containing 10 gene KCTD10 belongs to the polymerase delta-interacting protein 1 PDIP1 gene family. Mouse KCTD10 was thought to interact with proliferating cell nuclear antigen and the small subunit of polymerase Ỗ and to have roles in DNA repair DNA replication and cell-cycle control. To better understand the regulatory mechanism of KCTD10 expression we characterized the promoter of human KCTD10 containing a 639 bp fragment of the 5 -flanking region -609 30 . A primer extension assay identified the transcription start site as an A 63 bp upstream of the start codon. The promoter region contains neither a TATA box nor a CCAAT box but a CpG island was found near to the transcription start site. Deletion mutagenesis showed that the region from -108 to 30 was indispensable to the promoter activity and site-directed mutation analysis in this proximal promoter region of KCTD10 revealed two important transcription regulatory elements of .