tailieunhanh - Báo cáo y học: "Antibodies against the CB10 fragment of type II collagen in rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Antibodies against the CB10 fragment of type II collagen in rheumatoid arthritis. | Available online http content 6 5 R477 Research article Antibodies against the CB10 fragment of type II collagen in rheumatoid arthritis Andrew D Cook1 Robyn Gray1 John Ramshaw2 Ian R Mackay1 and Merrill J Rowley1 Department of Biochemistry and Molecular Biology Monash University Clayton Victoria Australia 2CSIRO Molecular Science Parkville Victoria Australia Corresponding author Merrill J Rowley Received 2 Apr 2004 Revisions requested 14 May 2004 Revisions received 11 Jun 2004 Accepted 18 Jun 2004 Published 11 Aug 2004 Arthritis Res Ther 2004 6 R477-R483 DOI ar1213 2004 Cook et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Open Access Abstract Antibodies against intact type II collagen CII are a feature of rheumatoid arthritis RA but have limited diagnostic value. Here we assess whether either of the two major cyanogen bromide fragments of CII namely CB10 or CB11 are more sensitive substrates for the detection of antibodies in RA. Cleavage of bovine CII with cyanogen bromide yielded CB10 and CB11 these were purified by column chromatography for use in an enzyme-linked immunosorbent assay. Serum antibodies were measured in patients with RA psoriatic arthritis PsA osteoarthritis OA and blood donors. Results were compared with those using intact CII. Antibodies against CB10 were found in as many as 88 of 96 patients with long-standing RA but only 12 of 33 patients with PsA 6 of 34 patients with OA and 3 of 93 control sera. Lower frequencies for these diseases were obtained on testing for antibodies against CB11 50 6 21 and 2 respectively. The sensitivity of detection in RA of antibodies against CB10 compared with antibodies against intact CII 88 versus 24 was not at the expense of specificity which remained high at 94 . .