tailieunhanh - Báo cáo y học: "Membrane-associated prostaglandin E synthase-1 is upregulated by proinflammatory cytokines in chondrocytes from patients with osteoarthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Membrane-associated prostaglandin E synthase-1 is upregulated by proinflammatory cytokines in chondrocytes from patients with osteoarthritis. | Available online http content 6 4 R355 Research article Membrane-associated prostaglandin E synthase-1 is upregulated by proinflammatory cytokines in chondrocytes from patients with osteoarthritis Fumiaki Kojima1 Hiroaki Naraba2 Satoshi Miyamoto3 Moroe Beppu3 Haruhito Aoki3 and Shinichi Kawai1 Open Access Institute of Medical Science St Marianna University School of Medicine Kawasaki Japan 2National Cardiovascular Center Research Institute Osaka Japan 3Department of Orthopedic Surgery St Marianna University School of Medicine Kawasaki Japan Corresponding author Shinichi Kawai s2kawai@ Received 27 Dec 2003 Revisions requested 21 Jan 2003 Revisions received 1 Apr 2004 Accepted 12 May 2004 Published 8 Jun 2004 Arthritis Res Ther 2004 6 R355-R365 DOI ar1195 2004 Kojima et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Prostaglandin E synthase PGES including isoenzymes of membrane-associated PGES mPGES -1 mPGES-2 and cytosolic PGES cPGES is the recently identified terminal enzyme of the arachidonic acid cascade. PGES converts prostaglandin PG H2 to PGE2 downstream of cyclooxygenase COX . We investigated the expression of PGES isoenzyme in articular chondrocytes from patients with osteoarthritis OA . Chondrocytes were treated with various cytokines and the expression of PGES isoenzyme mRNA was analyzed by the reverse transcription-polymerase chain reaction and Northern blotting whereas Western blotting was performed for protein expression. The subcellular localization of mPGES-1 was determined by immunofluorescent microscopy. Conversion of arachidonic acid or PGH2 to PGE2 was measured by enzyme-linked immunosorbent assay. Finally the expression of mPGES-1 protein in OA articular cartilage was assessed by immunohistochemistry. .

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