tailieunhanh - Báo cáo y học: "Towards in vivo application of RNA interference – new toys, old problems"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Towards in vivo application of RNA interference – new toys, old problems. | Arthritis Research Therapy Vol 6 No 2 Rutz and Scheffold Technology review Towards in vivo application of RNA interference - new toys old problems Sascha Rutz and Alexander Scheffold Deutsches Rheuma-Forschungszentrum Berlin Berlin Germany Corresponding author Alexander Scheffold e-mail scheffold@ Received 17 Dec 2003 Revisions requested 11 Feb 2004 Revisions received 25 Feb 2004 Accepted 26 Feb 2004 Published 10 Mar 2004 Arthritis Res Ther 2004 6 78-85 DOI ar1168 2004 BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 Abstract RNA interference RNAi is the sequence-specific degradation of mRNA by short double-stranded RNA molecules. The technology introduced only 5 years ago has stimulated many fantasies regarding the future of functional gene analysis and gene therapy. Given its ease of application its high efficiency and remarkable specificity RNAi holds great promise for broad in vitro and in vivo application in all areas of biomedicine. Despite its potential the major obstacle to the use of RNAi as for all previous gene silencing approaches is the need for efficient and sustained delivery of small interfering RNA into primary mammalian cells and specific targeting of particular cell types in vivo. Keywords functional genomics gene silencing primary mammalian cell small interfering RNA transfection Introduction In the postgenomic era it has become a major challenge to develop efficient reverse genetic approaches . from genotype to phenotype to evaluate the function of a vast number of newly identified genes. Furthermore specific silencing of disease-relevant genes . from tumours pathogens or inflammatory mediators is an interesting therapeutic strategy. In this respect RNA interference RNAi technology which allows targeted knockdown of individual genes by so-called small interfering RNAs siRNAs 1 has already opened up new avenues for functional analyses in vitro and holds great promise for analytical as well as therapeutic .