tailieunhanh - Báo cáo y học: "β Treatment with recombinant interferon-β reduces inflammation and slows cartilage destruction in the collagen-induced arthritis model of rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: β Treatment with recombinant interferon-β reduces inflammation and slows cartilage destruction in the collagen-induced arthritis model of rheumatoid arthritis. | Available online http content 6 3 R239 Research article Open Access Treatment with recombinant interferon-p reduces inflammation and slows cartilage destruction in the collagen-induced arthritis model of rheumatoid arthritis Judith van Holten1 Kris Reedquist1 Pascale Sattonet-Roche2 Tom JM Smeets1 Christine Plater-Zyberk2 Margriet J Vervoordeldonk1 and Paul P Tak1 Division of Clinical Immunology and Rheumatology Academic Medical Center University of Amsterdam The Netherlands 2Serono Pharmaceutical Research Institute Geneva Switzerland Corresponding author Paul P Tak e-mail Received 6 Nov 2003 Revisions requested 12 Dec 2003 Revisions received 18 Feb 2004 Accepted 19 Feb 2004 Published 23 Mar 2004 Arthritis Res Ther 2004 6 R239-R249 DOI ar1165 2004 van Holten et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract We investigated the therapeutic potential and mechanism of action of IFN-p protein for the treatment of rheumatoid arthritis RA . Collagen-induced arthritis was induced in DBA 1 mice. At the first clinical sign of disease mice were given daily injections of recombinant mouse IFN-p or saline for 7 days. Disease progression was monitored by visual clinical scoring and measurement of paw swelling. Inflammation and joint destruction were assessed histologically 8 days after the onset of arthritis. Proteoglycan depletion was determined by safranin O staining. Expression of cytokines receptor activator of NF-kB ligand and c-Fos was evaluated immunohisto-chemically. The IL-1-induced expression of IL-6 IL-8 and granulocyte macrophage-colony-stimulating factor GM-CSF was studied by ELISA in supernatant of RA and osteoarthritis fibroblast-like synoviocytes incubated with IFN-p. We also Keywords antibodies cytokines inflammation .