tailieunhanh - Báo cáo y học: "In vitro model for the analysis of synovial fibroblast-mediated degradation of intact cartilage"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: In vitro model for the analysis of synovial fibroblast-mediated degradation of intact cartilage. | Available online http content 11 1 R25 Research article In vitro model for the analysis of synovial fibroblast-mediated degradation of intact cartilage David Pretzel1 Dirk Pohlers1 Sonke Weinert1 2 and Raimund W Kinne1 Open Access Experimental Rheumatology Unit Department of Orthopedics University Hospital Jena Friedrich Schiller University Jena Klosterlausnitzer Strasse 81 Eisenberg D-07607 Germany 2Current address Experimental Cardiology Otto von Guericke University Magdeburg Leipziger Strasse 44 Magdeburg D-39120 Germany Corresponding author David Pretzel Received 4 Jun 2008 Revisions requested 24 Jul 2008 Revisions received 20 Jan 2009 Accepted 18 Feb 2009 Published 18 Feb 2009 Arthritis Research Therapy 2009 11 R25 doi ar2618 This article is online at http content 11 1 R25 2009 Pretzel et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Activated synovial fibroblasts are thought to play a major role in the destruction of cartilage in chronic inflammatory rheumatoid arthritis RA . However profound insight into the pathogenic mechanisms and the impact of synovial fibroblasts in the initial early stages of cartilage destruction is limited. Hence the present study sought to establish a standardised in vitro model for early cartilage destruction with native intact cartilage in order to analyse the matrix-degrading capacity of synovial fibroblasts and their influence on cartilage metabolism. Methods A standardised model was established by co-culturing bovine cartilage discs with early-passage human synovial fibroblasts for 14 days under continuous stimulation with TNF-a IL-1P or a combination of TNF-a IL-1p. To assess