tailieunhanh - Báo cáo y học: "Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin. | Arthritis Research Therapy Vol 6 No 2 Vossenaar et al. Research article Open Access Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin Erik R Vossenaar1 Normand Després2 Elvy Lapointe2 Annemarie van der Heijden1 Maximillian Lora2 Tatsuo Senshu3 Walther J van Venrooij1 and Henri A Ménard2 1 Department of Biochemistry University of Nijmegen Nijmegen The Netherlands 2Division of Rheumatology McGill University Health Centre Montreal Canada 3Graduate School of Integrated Science Yokohama City University Yokohama Japan Corresponding author Erik R Vossenaar e-mail Received 15 Dec 2003 Revisions requested 5 Jan 2004 Revisions received 16 Jan 2004 Accepted 19 Jan 2004 Published 5 Feb 2004 Arthritis Res Ther 2004 6 R142-R150 DOI ar1050 2004 Vossenaar et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Antibodies directed to the Sa antigen are highly specific for rheumatoid arthritis RA and can be detected in approximately 40 of RA sera. The antigen a doublet of protein bands of about 50 kDa is present in placenta and in RA synovial tissue. Although it has been stated that the Sa antigen is citrullinated vimentin experimental proof for this claim has never been published. In this study we investigated the precise nature of the antigen. Peptide sequences that were obtained from highly purified Sa antigen were unique to vimentin. Recombinant vimentin however was not recognized by anti-Sa reference sera. In vivo vimentin is subjected to various post-translational modifications including citrullination. Since antibodies to citrullinated proteins are known to be highly specific for RA we investigated whether Sa is citrullinated and found that Sa indeed is citrullinated vimentin. Anti-Sa .