tailieunhanh - Báo cáo y học: "Giantin is the major Golgi autoantigen in human anti-Golgi complex sera"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Giantin is the major Golgi autoantigen in human anti-Golgi complex sera. | Available online http content 6 2 R95 Research article Open Access Giantin is the major Golgi autoantigen in human anti-Golgi complex sera Kazuhisa Nozawa1 Marvin J Fritzler2 Carlos A von Muhlen3 and Edward K L Chan1 Department of Oral Biology University of Florida College of Dentistry Gainesville Florida USA 2Department of Biochemistry and Molecular Biology University of Calgary Calgary Alberta Canada 3Department of Internal Medicine Hospital São Lucas Pontificia Universidade Católica do Rio Grande do Sul Porto Alegre Brazil Corresponding author Edward K Chan e-mail echan@ Received 29 Sep 2003 Revisions requested 24 Oct 2003 Revisions received 19 Nov 2003 Accepted 27 Nov 2003 Published 15 Dec 2003 Arthritis Res Ther 2004 6 R95-R102 DOI ar1035 2004 Nozawa et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Anti-Golgi complex antibodies AGAs are primarily associated with systemic lupus erythematosus and Sjogren s syndrome. Here we report on the immunoreactivity of AGAs against five Golgi autoantigens giantin golgin-245 golgin-160 golgin-95 GM130 and golgin-97 and provide data from epitope mapping on the most common Golgi autoantigen namely giantin. A total of 80 human sera containing AGAs as defined by indirect immunofluorescence on HEp-2 cells were analyzed by ELISA using recombinant autoantigens and immunoprecipitation. The proportion of AGA sera that reacted with the five Golgi autoantigens was correlated with the molecular mass of the Golgi antigens. Autoantibodies to giantin the largest Golgi autoantigen were the predominant AGAs being found in 50 of the AGA sera. Epitope mapping of giantin was performed using six recombinant fragments spanning the entire protein. Antigiantin-positive sera .