tailieunhanh - Báo cáo y học: " Egr-1 inhibits the expression of extracellular matrix genes in chondrocytes by TNF-induced MEK/ERK signalling"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Egr-1 inhibits the expression of extracellular matrix genes in chondrocytes by TNF -induced MEK/ERK signalling. | Available online http content 11 1 R8 Research article Egr-1 inhibits the expression of extracellular matrix genes in chondrocytes by TNFa-induced MEK ERK signalling Jason S Rockel1 2 Suzanne M Bernier1 2 and Andrew Leask1 3 Open Access Canadian Institutes of Health Research Group in Skeletal Development and Remodeling Schulich School of Medicine Dentistry The University of Western Ontario London Ontario N6A 5C1 Canada 2Department of Anatomy and Cell Biology Schulich School of Medicine Dentistry The University of Western Ontario London Ontario N6A 5C1 Canada 3Division of Oral Biology Schulich School of Medicine Dentistry The University of Western Ontario London Ontario N6A 5C1 Canada Corresponding author Andrew Leask Received 10 Nov 2008 Revisions requested 5 Dec 2008 Revisions received 8 Dec 2008 Accepted 14 Jan 2009 Published 14 Jan 2009 Arthritis Research Therapy 2009 11 R8 doi 1 ar2595 This article is online at http content 11 1 R8 2009 Rockel et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction TNFa is increased in the synovial fluid of patients with rheumatoid arthritis and osteoarthritis. TNFa activates mitogen-activated kinase kinase MEK extracellular regulated kinase ERK in chondrocytes however the overall functional relevance of MEK ERK to TNFa-regulated gene expression in chondrocytes is unknown. Methods Chondrocytes were treated with TNFa with or without the MEK1 2 inhibitor U0126 for 24 hours. Microarray analysis and real-time PCR analyses were used to identify genes regulated by TNFa in a MEK1 2-dependent fashion. Promoter reporter immunoblot and electrophoretic mobility shift assays were used to identify