tailieunhanh - Báo cáo y học: "Microarray analysis of gene expression in lupus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Microarray analysis of gene expression in lupus. | Available online http content 5 6 279 Review Microarray analysis of gene expression in lupus Mary K Crow1 and Jay Wohlgemuth2 1Mary Kirkland Center for Lupus Research Hospital for Special Surgery New York NY USA 2Expression Diagnostics Inc South San Francisco CA USA Corresponding author Mary K Crow e-mail crowm@ Received 26 Aug 2003 Revisions requested 5 Sep 2003 Revisions received 22 Sep 2003 Accepted 1 Oct 2003 Published 13 Oct 2003 Arthritis Res Ther 2003 5 279-287 DOI ar1015 2003 BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 Abstract Recent advances in the study of global patterns of gene expression with the use of microarray technology coupled with data analysis using sophisticated statistical algorithms have provided new insights into pathogenic mechanisms of disease. Complementary and reproducible data from multiple laboratories have documented the feasibility of analysis of heterogeneous populations of peripheral blood mononuclear cells from patients with rheumatic diseases through use of this powerful technology. Although some patterns of gene expression including increased expression of immune system cell surface activation molecules confirm previous data obtained with other techniques some novel genes that are differentially expressed have been identified. Most interesting is the dominant pattern of interferon-induced gene expression detected among blood mononuclear cells from patients with systemic lupus erythematosus and juvenile dermatomyositis. These data are consistent with longstanding observations indicating increased circulating interferon-a in the blood of patients with active lupus but draw attention to the dominance of the interferon pathway in the hierarchy of gene expression pathways implicated in systemic autoimmunity. Keywords gene expression interferon microarray statistical algorithms systemic lupus erythematosus Introduction the dawning of the microarray era The concept that the .