tailieunhanh - Báo cáo y học: "Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats. | Available online http content 5 6 R361 Research article Open Access Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats Lena Wester1 Dirk Koczan1 Jens Holmberg2 Peter Olofsson2 Hans-Jurgen Thiesen1 Rikard Holmdahl2 and Saleh Ibrahim1 institute fur Immunologie Universitat Rostock Rostock Germany 2Medical Inflammation Research Biomedical Center Lund University Lund Sweden Corresponding author Lena Wester e-mail Received 28 Apr 2003 Revisions requested 10 Jul 2003 Revisions received 21 Jul 2003 Accepted 4 Aug 2003 Published 2 Oct 2003 Arthritis Res Ther 2003 5 R361-R372 DOI ar993 2003 Wester et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Arthritis susceptibility genes were sought by analysis of differential gene expression between pristane-induced arthritis PIA -susceptible DA rats and PIA-resistant E3 rats. Inguinal lymph nodes of naive animals and animals 8 days after pristane injection were analyzed for differential gene expression. mRNA expression was investigated by microarray and real-time PCR and protein expression was analyzed by flow cytometry or ELISA. Twelve genes were significantly differentially expressed when analyzed by at least two independent methods and an additional five genes showed a strong a tendency toward differential expression. In naive DA rats IgE the bone marrow stromal cell antigen 1 Bst1 and the MHC class II p-chain MhcII were expressed at a higher level and the immunoglobulin kappa chain IgK was expressed at a lower level. In pristane-treated DA rats the MHC class II p-chain gelatinase B Mmp9 and the protein tyrosine phosphatase CL100 Ptpn16 were expressed at a higher level whereas immunoglobulins .