tailieunhanh - Báo cáo y học: "A comparative study of the inhibitory effects of interleukin-1 receptor antagonist following administration as a recombinant protein or by gene transfer."

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: A comparative study of the inhibitory effects of interleukin-1 receptor antagonist following administration as a recombinant protein or by gene transfer. | Available online http content 5 5 R301 Research article Open Access A comparative study of the inhibitory effects of interleukin-1 receptor antagonist following administration as a recombinant protein or by gene transfer Jean-Noel Gouze1-3 Elvire Gouze1 2 Glyn D Palmer1 2 Victor S Liew1 2 Arnulf Pascher1 2 Oliver B Betz1 2 Thomas S Thornhill2 Christopher H Evans1 2 Alan J Grodzinsky3 and Steven C Ghivizzani1 2 1Center for Molecular Orthopaedics Brigham and Women s Hospital Harvard Medical School Boston Massachusetts USA 2Department of Orthopedic Surgery Brigham and Women s Hospital Harvard Medical School Boston Massachusetts USA 3Center for Biomedical Engineering Massachusetts Institute of Technology Cambridge Massachusetts USA Correspondence Steven C Ghivizzani e-mail sghivizzani@ Received 6 Nov 2002 Revisions requested 13 Feb 2003 Revisions received 23 Jun 2003 Accepted 1 Jul 2003 Published 1 Aug 2003 Arthritis Res Ther 2003 5 R301-R309 DOI ar795 2003 Gouze et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Anakinra the recombinant form of IL-1 receptor antagonist IL-1Ra has been approved for clinical use in the treatment of rheumatoid arthritis as the drug KineretTM but it must be administered daily by subcutaneous injection. Gene transfer may offer a more effective means of delivery. In this study using prostaglandin E2 production as a measure of stimulation we quantitatively compared the ability of anakinra as well as that of IL-1Ra delivered by gene transfer to inhibit the biologic actions of IL-1p. Human synovial fibroblast cultures were incubated with a range of doses of anakinra or HIG-82 cells genetically modified to constitutively express IL-1Ra. The cultures were then .